Purification and activity assays of N-terminal acetyltransferase D.

Yi-Hsun Ho, Emma K Seipp, Rong Huang

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Article in Methods in enzymology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

3 authors.

Yi-Hsun HoBorch Department of Medicinal Chemistry and Molecular Pharmacology, Purdue Institute for Drug Discovery, Purdue University Center for Cancer Research, Purdue University, West Lafayette, IN, United States.

Emma K SeippBorch Department of Medicinal Chemistry and Molecular Pharmacology, Purdue Institute for Drug Discovery, Purdue University Center for Cancer Research, Purdue University, West Lafayette, IN, United States.

Rong HuangBorch Department of Medicinal Chemistry and Molecular Pharmacology, Purdue Institute for Drug Discovery, Purdue University Center for Cancer Research, Purdue University, West Lafayette, IN, United States. Electronic address: huang-r@purdue.edu.

Funding

Discovery of small molecule inhibitors for protein N-terminal acetyltransferase DR01CA258887 · NCI · PURDUE UNIVERSITY · PI HUANG, RONG · 2022 to 2025

$2.1M

NCI NIH HHS R01 CA258887

6 · The paper itself

Abstract

α-N-terminal acetyltransferase D (NatD) specifically acetylates the α-amine of the N-terminal SGRGK motif on histones H4 and H2A, a modification crucial for regulating gene expression. Thus, assays for the characterization of NatD-mediated acetylation is essential to study its functions and discover inhibitors. Here we present two methods for characterizing NatD-mediated acetylation: fluorescence-based and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) assays. We describe detailed procedures to characterize enzymatic kinetics and assess inhibitor activity for NatD. Additionally, we discuss the advantages and limitations of each approach compared to other in vitro acetyltransferase assays and highlight their broader applicability for evaluating N-terminal acetylation.

Indexed as

Enzyme AssaysN-Terminal AcetyltransferasesAcetylationHistonesHumansKineticsRecombinant ProteinsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationHistonesN-Terminal AcetyltransferasesRecombinant Proteinsacetylationacetyltransferasefluorescence assayMALDI-MSNα-acetylation

Identifiers

PMID40887159

DOI10.1016/bs.mie.2025.06.019

PMCPMC12498240

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