Evidence map›Paper›PMID 40886281›Full record

ReviewAdvances in experimental medicine and biology2025

CircRNA Synthesis and Applications.

Adam Greasley, Shuailong Li, KeXiang Liu, Xiufen Zheng

Abstract readReview
PubMed Publisher
In one paragraph

Review in Advances in experimental medicine and biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Adam GreasleyDepartment of Pathology and Laboratory Medicine, Western University, London, ON, Canada.
Shuailong LiDepartment of Pathology and Laboratory Medicine, Western University, London, ON, Canada.
KeXiang LiuDepartment of Cardiovascular Surgery, The Second Hospital of Jilin University, Changchun, China.
Xiufen ZhengDepartment of Pathology and Laboratory Medicine, Western University, London, ON, Canada. xzheng26@uwo.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Circular RNA (circRNA) is becoming an increasingly popular field of study and topic of interest. With that, there is a renewed and heightened interest in generating circular RNA for both research and clinical use. CircRNA generation began in the 1990s using in vitro chemical ligation and has since expanded out to include in vitro enzymatic synthesis along with in vivo vector-based systems. Each of these methods has distinct advantages, limitations, and different function 1ality depending on application. Throughout this chapter, we explore and summarize different methods for generating pure circRNA constructs that can be readily utilized for different applications. We highlight the steps and aspects to be considered when selecting the method to synthesize circRNA based on yield, purity, and application. Overall, this chapter aims to provide the reader with an understanding of the current methods and applications of synthesizing circRNA both in vivo and in vitro for use in research and clinical settings.

Indexed as

RNARNA, CircularAnimalsHumansRNARNA, CircularcircRNA vectorEnzymatic ligationIn vitro transcriptionPermuted intron–exon

Identifiers

PMID40886281

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.