ArticleMethods in molecular biology (Clifton, N.J.)2025
Isolation of Germ Cell Populations from Mouse Testis by Flow Cytometry.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Mammalian spermatogenesis is an intricate process resulting in the formation of male haploid gametes. In order to attain a better understanding of the complexity of this process, the development of a reproducible cytometry method to isolate germ cells is much needed. Here, we describe a reproducible cytometry protocol to isolate germ cells from adult mouse testis. We paid special attention to the optimization of what are considered the most critical steps, including testis disaggregation, antibody titration, cell staining, and germ cell discrimination by FACS.
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