Evidence map›Paper›PMID 40884663›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Single-Molecule Studies with AFM and High-Speed AFM: From Nucleosomes to Chromosomes.

Daniël P Melters, Keir C Neuman, Yamini Dalal

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Daniël P MeltersLaboratory of Receptor Biology and Gene Expression, National Cancer Institute, Center for Cancer Research, Bethesda, MD, USA. daniel.melters@nih.gov.
Keir C NeumanLaboratory of Single Molecule Biophysics, National Heart, Lung, and Blood Institute, Bethesda, MD, USA. neumankc@nih.gov.
Yamini DalalLaboratory of Receptor Biology and Gene Expression, National Cancer Institute, Center for Cancer Research, Bethesda, MD, USA. dalaly@mail.nih.gov.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Atomic force microscopy (AFM) is a single-molecule imaging technique invented in 1985. Development of high-speed AFM (HS-AFM) in 2001 now allows for the direct visualization of the dynamics and kinetics of biological molecules. In this chapter, we describe methods to both reconstitute in vitro and extract in vivo chromatin for air mode AFM and HS-AFM studies. With these methods, sub-nanometer static and time-resolved topographical measurements of nucleosome and chromatin conformations and dynamics can be obtained. Furthermore, we discuss how HS-AFM data can be analyzed to obtain quantitative measures of nucleosome dynamics.

Indexed as

ChromosomesMicroscopy, Atomic ForceNucleosomesSingle Molecule ImagingAnimalsChromatinChromatinNucleosomesAtomic force microscopyChromatinChromosomeDiffusion constantHigh-speed AFMMean square displacement (MSD)Nucleosome

Identifiers

PMID40884663

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.