ArticleMethods in molecular biology (Clifton, N.J.)2025
Oligopaint FISH to Study Chromosomal Architecture and Structural Variations.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- A scaffolding element rewires local 3D chromatin architecture during differentiation.Nature communications · 2026Article
- Epistemological parameters in the recent era of nuclear organization and function.Molecular biology of the cell · 2026Review
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Authors and funding
6 authors.
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Abstract
Three-dimensional DNA fluorescent in situ hybridization (3D-FISH) has been extensively used for the study of chromosome organization, from large-scale chromosome territories to finer sub-megabase structures, such as topologically associating domains (TADs) or internal TAD features such as chromatin loops. The development of oligo-based technology has rendered this technique highly versatile and allowed to study chromosomal structures at different scale levels, as well as structural variations such as deletions, duplications, or translocations, as those occurring in cancer and chromothripsis. Here, we present a comprehensive Oligopaint FISH protocol, from probe design and production to FISH, microscopy, and specific analysis that can be used with various cell lines.
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