ArticlePhysiologia plantarum
SlSPL15: A Negative Regulator Targeted by SlmiR156a Participates in Regulating the Light-Induced Anthocyanin Biosynthesis of Aft Tomato Fruits.
Article in Physiologia plantarum. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Anthocyanins are crucial compounds known for their antioxidant and health benefits. The Aft tomato (Solanum lycopersicum) line LA1996 can generate anthocyanins in fruits upon light exposure. Despite the identification of various regulatory genes, such as microRNAs and transcription factors involved in anthocyanin biosynthesis across different plant species, the function of the miR156/SPL module in Aft tomato fruit pigmentation is not well understood. In this research, 17 SlSPL family genes of Aft tomato were classified into six clades. SlSPL15 (Solyc10T002263.1) was grouped in Clade V, with AtSPL9, which is known to be involved in anthocyanin biosynthesis in Arabidopsis. Moreover, an inverse relationship between SlSPL15 and miR156a expression in mature green (MG) stage fruits was shown by quantitative RT-PCR analysis. Transient expression analysis of luciferase confirmed that SlSPL15 transcripts are degraded by SlmiR156a. Furthermore, overexpression of SlSPL15 in Aft tomato reduced the anthocyanin accumulation in MG fruits. In SlSPL15 overexpressed tomato plants, the transcription level of SlSPL15 was elevated compared to that in wild-type fruits, whereas the expression of SlPAL, SlCHS1, SlDFR, SlF3H, SlF3'5'H, and SlANS was reduced. Additionally, the expression of light-responsive regulatory genes SlHY5, SlAN2-like, and SlMYB12 in the anthocyanin biosynthetic pathway was also reduced in light-exposed fruits of 35S:SlSPL15 plants. Subcellular localization analysis verified that SlSPL15 is localized in the nucleus, while yeast two-hybrid assays demonstrated its interaction with SlAN2-like, a part of the MBW complex that participates in regulating anthocyanin biosynthesis in Aft tomato fruits. The findings expand our comprehension of the roles of SlSPL15, targeted by SlmiR156a, in regulating light-induced anthocyanin accumulation in tomatoes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.