Evidence map›Paper›PMID 40883657›Full record

ArticleInsect science2026

The shibire

Anzu Okada, Mamoru Okamoto, Thu N M Nguyen, Elisabeth Fung, Han Nguyen, Peter Crisp, Amanda Choo, Simon W Baxter

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Insect science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Anzu OkadaSchool of Biological Sciences, The University of Adelaide, Adelaide, South Australia, Australia.
Mamoru OkamotoSchool of Biological Sciences, The University of Adelaide, Adelaide, South Australia, Australia.
Thu N M NguyenSchool of BioSciences, The University of Melbourne, Melbourne, Victoria, Australia.
Elisabeth FungSouth Australian Research and Development Institute (SARDI), Urrbrae, South Australia, Australia.
Han NguyenDepartment of Applied Biosciences, Macquarie University, Macquarie Park, New South Wales, Australia.
Peter CrispSouth Australian Research and Development Institute (SARDI), Urrbrae, South Australia, Australia.
Amanda ChooSchool of Biological Sciences, The University of Adelaide, Adelaide, South Australia, Australia.
Simon W BaxterSchool of BioSciences, The University of Melbourne, Melbourne, Victoria, Australia.ORCID https://orcid.org/0000-0001-5773-6578

Funding

International Atomic Energy Agency Coordinated Research Project D44003International Atomic Energy Agency Coordinated Research Project HG14033This project was part of the National SITplus program and supported by Horticulture Innovation using the research and development levy funds from the vegetable, apple and pear, citrus, strawberry, table grape, cherry and summerfruit industries, with co-investment from South Australian Research and Development Institute (SARDI) and Primary Industries and Regions South Australia (PIRSA), and contributions from the Australian Government.
6 · The paper itself

Abstract

Bactrocera tryoni, the Queensland fruit fly, is among the most damaging insect pests to the Australian horticultural industry as larvae infest ripening fruits or vegetables prior to harvest. Genetic biocontrol using Sterile Insect Technique (SIT) programs have been used to successfully suppress populations, via mass release of factory-reared sterile males that mate with wild females. Bi-sex flies are currently used for releases, although the efficiency of these control programs could be improved through using genetic sexing strains that eliminate females early during development, as they are not required for SIT. Here we used CRISPR/Cas9 mutagenesis to modify two nucleotides in the B. tryoni gene shibire, which created a proline to serine amino acid substitution and produced a temperature sensitive phenotype. Shibire is an essential GTPase required in endocytosis and synaptic vesicle recycling, and classical mutagenic screens in the vinegar fly Drosophila melanogaster previously identified temperature sensitive alleles including shi

Indexed as

Insect ProteinsTephritidaeAnimalsCRISPR-Cas SystemsFemaleLarvaMaleMutationPhenotypePupaTemperatureInsect ProteinsCRISPR/Cas9genetic sexing strainsterile insect technique

Identifiers

PMID40883657
PMCPMC13087831

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.