Evidence map›Paper›PMID 40883309›Full record

ArticleNature communications2025

Characterization of PROTAC specificity and endogenous protein interactomes using ProtacID.

Suman Shrestha, Matthew E R Maitland, Laili Jing, Shili Duan, David Y Nie, Jonathan St-Germain, Michael Kanaris, Dalia Barsyte-Lovejoy, Cheryl H Arrowsmith, Brian Raught

Abstract read
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In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Suman ShresthaPrincess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada.ORCID http://orcid.org/0000-0002-7011-7496
Matthew E R MaitlandPrincess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada.
Laili JingPrincess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada.
Shili DuanPrincess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada.
David Y NiePrincess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada.
Jonathan St-GermainPrincess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada.
Michael KanarisStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.ORCID http://orcid.org/0009-0003-0760-1650
Dalia Barsyte-LovejoyStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.ORCID http://orcid.org/0000-0002-6560-9621
Cheryl H ArrowsmithPrincess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada. cheryl.arrowsmith@uhn.ca.ORCID http://orcid.org/0000-0002-4971-3250
Brian RaughtPrincess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada. brian.raught@uhn.ca.ORCID http://orcid.org/0000-0001-6145-4776

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here we describe ProtacID, a flexible BioID (proximity-dependent biotinylation)-based approach to identify PROTAC-proximal proteins in living cells. ProtacID analysis of VHL- and CRBN-recruiting PROTACs targeting a number of different proteins (localized to chromatin or cellular membranes, and tested across six different human cell lines) demonstrates how this technique can be used to validate PROTAC degradation targets and identify non-productive (i.e. non-degraded) PROTAC-interacting proteins, addressing a critical need in the field of PROTAC development. We also demonstrate that ProtacID can be used to characterize native, endogenous multiprotein complexes without the use of antibodies, or modification of the protein of interest with epitope tags or biotin ligase tagging.

Indexed as

Adaptor Proteins, Signal TransducingUbiquitin-Protein LigasesVon Hippel-Lindau Tumor Suppressor ProteinBiotinylationCell LineHEK293 CellsHumansProtein BindingProteolysisAdaptor Proteins, Signal TransducingCRBN protein, humanUbiquitin-Protein LigasesVHL protein, humanVon Hippel-Lindau Tumor Suppressor Protein

Identifiers

PMID40883309
PMCPMC12397304

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.