Evidence map›Paper›PMID 40881988›Full record

ArticleRoyal Society open science2025

PNGaseL from

Cassie R Bakshani, Paulina A Urbanowicz, Conchi Badia Tortosa, Javier M Melo Diaz, Magdalena Kujawska, Taiwo O Ojuri, Lindsay J Hall, Daniel I R Spencer, David N Bolam, Lucy I Crouch

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Royal Society open science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. PNGaseL fromRoyal Society open science · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Cassie R BakshaniDepartment of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, Institute of Microbiology and Infection, University of Birmingham, Birmingham, England, UK.
Paulina A UrbanowiczLudger Ltd, Abingdon, England, UK.
Conchi Badia TortosaLudger Ltd, Abingdon, England, UK.
Javier M Melo DiazLudger Ltd, Abingdon, England, UK.
Magdalena KujawskaDepartment of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, Institute of Microbiology and Infection, University of Birmingham, Birmingham, England, UK.
Taiwo O OjuriDepartment of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, Institute of Microbiology and Infection, University of Birmingham, Birmingham, England, UK.
Lindsay J HallDepartment of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, Institute of Microbiology and Infection, University of Birmingham, Birmingham, England, UK.
Daniel I R SpencerLudger Ltd, Abingdon, England, UK.
David N BolamBiosciences Institute, Newcastle University, Newcastle upon Tyne, England, UK.
Lucy I CrouchDepartment of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, Institute of Microbiology and Infection, University of Birmingham, Birmingham, England, UK.ORCID https://orcid.org/0000-0003-1479-3534

Funding

Wellcome Trust
6 · The paper itself

Abstract

Peptide:N-glycosidases (PNGases) are used by a wide range of organisms to remove N-glycan structures from proteins for use as either nutrients or in glycoprotein processing. PNGaseF is the most well-characterized enzyme of this family and is widely used in glycobiology to allow study of the N-glycome of a specific protein, cell and tissues, for instance. Despite this, PNGaseF has limitations in the types of N-glycan structures it can target. In this study, we explored the specificities of six uncharacterized PNGases selected from diverse parts of the PNGaseF superfamily. One of these enzymes, PNGaseL from

Indexed as

chromatographycrystal structuresglycobiologymodellingN-glycansnutrient acquisitionPNGase

Identifiers

PMID40881988
PMCPMC12381499

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.