Evidence map›Paper›PMID 40880634›Full record

ArticleFrontiers in cellular and infection microbiology2025

Development and evaluation of a multiplex molecular point-of-care assay for direct identification of

Qiao-Lian Yi, Yun Wu, Shuang He, Meng-Li Feng, Xiao-Yu Liu, Xin-Zhu Zhou, Hao-Tian Gao, Yu-Fan Zhang, Qi-Wen Yang, Ying-Chun Xu

Abstract readEvaluation Study
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Qiao-Lian Yi *Department of Laboratory Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Yun Wu *Department of Laboratory Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Shuang HeR&D Department Genewise Bio Co., Ltd, Beijing, China.
Meng-Li FengR&D Department Genewise Bio Co., Ltd, Beijing, China.
Xiao-Yu LiuDepartment of Laboratory Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Xin-Zhu ZhouDepartment of Laboratory Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Hao-Tian GaoDepartment of Laboratory Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Yu-Fan ZhangR&D Department Genewise Bio Co., Ltd, Beijing, China.
Qi-Wen YangDepartment of Laboratory Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Ying-Chun XuDepartment of Laboratory Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: This study aimed to establish a multiplex molecular point-of-care assay called Methods: The study was divided into two stages: a pilot study to establish the methodology and a clinical validation study to evaluate its performance. In the pilot study, we established the Results: Of the 149 positive clinical mycobacterial cultures analyzed, 136 were within the designed targets. Among these 136 cultures, 133 samples were correctly identified by Conclusions: This study demonstrates that

Indexed as

Molecular Diagnostic TechniquesMultiplex Polymerase Chain ReactionMycobacterium Infections, NontuberculousMycobacterium tuberculosisNontuberculous MycobacteriaPoint-of-Care SystemsTuberculosisDNA, BacterialHumansPilot ProjectsReproducibility of ResultsRNA, Ribosomal, 16SSensitivity and SpecificityDNA, BacterialRNA, Ribosomal, 16Smolecular diagnosisMycobacterium tuberculosisnon-tuberculous mycobacteriapathogen identificationpoint-of-care testing

Identifiers

PMID40880634
PMCPMC12380709

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.