Evidence map›Paper›PMID 40879977›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Enzymatic Synthesis of Inositol Pyrophosphates.

Simon M Bartsch, Dorothea Fiedler

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Simon M BartschLeibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany.
Dorothea FiedlerLeibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany. Fiedler@fmp-berlin.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The availability of well-defined and pure PP-InsP substrates in high quantities is pivotal to study their molecular mechanisms by means of biochemical or biophysical approaches. Here, we present a workflow employing enzymatic synthesis that allows for the quick and easy synthesis and isolation of mammalian PP-InsP isomers. With the respective enzymes, inositol pyrophosphates can be generated in a single reaction step. Isolation and purification of the desired products does not require special laboratory equipment and can be achieved by precipitation of PP-InsPs as magnesium salts. Having access to PP-InsPs will aid the field in discovering and characterizing their molecular mechanisms and binding modes.

Indexed as

Inositol PhosphatesAnimalsHumansInositol PhosphatesEnzymatic synthesisInositol pyrophosphatesIon-exchangeIsomer selectivityPrecipitationScalable synthesis

Identifiers

PMID40879977

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.