Evidence map›Paper›PMID 40879976›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Analysis of Inositol Phosphates via Polyacrylamide Gel Electrophoresis (PAGE).

Valeria Fedeli, Adolfo Saiardi

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Valeria FedeliLaboratory for Molecular Cell Biology, University College London, London, UK.
Adolfo SaiardiLaboratory for Molecular Cell Biology, University College London, London, UK. a.saiardi@ucl.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Polyacrylamide gel electrophoresis (PAGE) is a versatile technique widely used in molecular biology for the separation of biomolecules based on size and charge. While it is traditionally applied to proteins and nucleic acids, recently, PAGE has been adapted for the analysis of inositol polyphosphates and their pyrophosphate derivatives, which play multiple roles in cellular signaling. Studying these molecules presents analytical challenges due to their small size, high charge density, and low concentrations in biological samples. In this work, we describe an optimized PAGE protocol for the separation and detection of inositol polyphosphates and inositol pyrophosphate. The method uses high-concentration polyacrylamide gels and toluidine blue staining to achieve sensitive detection at nanomolar levels. The simplicity and cost-efficiency of the approach make it accessible to most laboratories. This method provides a reliable tool for investigating inositol-phosphate-based signaling pathways and their involvement in cellular processes.

Indexed as

Electrophoresis, Polyacrylamide GelInositol PhosphatesInositol PhosphatesDiphosphoinositol pentakisphosphateInositol hexakisphosphateInositol phosphatesInositol pyrophosphateIP6KsPolyacrylamide gel electrophoresis

Identifiers

PMID40879976

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.