Evidence map›Paper›PMID 40879914›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Utilizing Neonatal Tracheal Aspirate-Derived Bronchial Basal Stem Cells to Model Respiratory Syncytial Virus Infection.

Caiqi Zhao, Gaurang M Amonkar, Paul H Lerou, Xingbin Ai

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Caiqi ZhaoDepartment of Pediatrics, Division of Newborn Medicine, Massachusetts General Hospital, Boston, MA, USA.
Gaurang M AmonkarDepartment of Pediatrics, Division of Newborn Medicine, Massachusetts General Hospital, Boston, MA, USA.
Paul H LerouDepartment of Pediatrics, Division of Newborn Medicine, Massachusetts General Hospital, Boston, MA, USA.
Xingbin AiDepartment of Pediatrics, Division of Newborn Medicine, Massachusetts General Hospital, Boston, MA, USA. xai@mgh.harvard.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Respiratory syncytial virus (RSV) infection is a primary cause of bronchiolitis and hospitalization in infants and is associated with significant mortality and morbidity. To model RSV infection in infant bronchial epithelium, we developed a robust protocol for bronchial basal stem cell (BSC) derivation from tracheal aspirates (TAs) of intubated neonatal patients. TA BSCs can be expanded while retaining the ability to differentiate into all the major types of bronchial epithelial cells in air-liquid interface (ALI), making them an invaluable source to generate functional bronchial epithelium of infants. This protocol outlines the specific steps in utilizing TA BSCs to model RSV infection of infant bronchial epithelium in ALI culture.

Indexed as

BronchiRespiratory Syncytial VirusesRespiratory Syncytial Virus InfectionsStem CellsTracheaCell Culture TechniquesCell DifferentiationCells, CulturedEpithelial CellsHumansInfant, NewbornRespiratory MucosaAir-liquid interface (ALI)Airway epithelial cells (AEC)Basal stem cell (BSC)InfantRespiratory syncytial virus (RSV)Tracheal aspirate (TA)

Identifiers

PMID40879914

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.