Evidence map›Paper›PMID 40879912›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Bisulfite Sequencing to Map 5-Methylcytosine Sites in Human Respiratory Syncytial Virus RNA.

Li-Sheng Zhang, Yuexiu Zhang, Amit Sharma, Chuan He, Jianrong Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Li-Sheng ZhangDepartment of Chemistry, Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, University of Chicago, Chicago, IL, USA.
Yuexiu ZhangDepartment of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA.
Amit SharmaDepartment of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
Chuan HeDepartment of Chemistry, Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, University of Chicago, Chicago, IL, USA.
Jianrong LiDepartment of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA. li.926@osu.edu.

Funding

Role of the Non-canonical Inflammasome in SARS-CoV-2-mediated Pathology and CoagulopathyP01AI175399 · NIAID · OHIO STATE UNIVERSITY · PI Amal O Amer, Estelle A Cormet-Boyaka · 2024 to 2026
$12.0M
Messenger RNA Capping and Methylation in PneumovirusesR01AI090060 · NIAID · OHIO STATE UNIVERSITY · PI LI, JIANRONG · 2011 to 2023
$4.0M
Determinants of retroviral replication in non-native hosts for modeling HIV infectionR01AI172615 · NIAID · OHIO STATE UNIVERSITY · PI Amit Sharma · 2023 to 2026
$3.1M
NIAID NIH HHS P01 AI175399NIAID NIH HHS R01 AI090060NIAID NIH HHS R01 AI172615
6 · The paper itself

Abstract

Viral epigenetic modification is an emerging field. As an obligate intracellular parasite, viral genetic material is exposed to host RNA-modifying enzymes. Currently, more than 180 types of RNA modifications have been identified. Like all non-segmented negative-sense RNA viruses, human respiratory syncytial virus (RSV) infection produces three types of RNA: genome and antigenome that are encapsidated by viral nucleocapsid and 10 mRNAs that are capped, methylated, and polyadenylated. Recently, the RSV genome, antigenome, and mRNAs have been shown to be modified by N6-methyladenosine (m

Indexed as

5-MethylcytosineRespiratory Syncytial Virus, HumanRNA, ViralSequence Analysis, RNASulfitesA549 CellsGenome, ViralHumansRespiratory Syncytial Virus InfectionsRNA Processing, Post-Transcriptional5-Methylcytosinehydrogen sulfiteRNA, ViralSulfites5-methylcytosineBisulfite sequencingRespiratory syncytial virus (RSV)RNA modification

Identifiers

PMID40879912
PMCPMC13292810

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.