Evidence map›Paper›PMID 40879908›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Single-Particle Electron Microscopy Analysis of Respiratory Syncytial Virus Protein Complexes.

Dongdong Cao, Yunrong Gao, Zhenhang Chen, Natalie Horgan, Bo Liang

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Dongdong CaoDepartment of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA.
Yunrong GaoDepartment of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA.
Zhenhang ChenDepartment of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA.
Natalie HorganDepartment of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA.
Bo LiangDepartment of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA. bo.liang@emory.edu.

Funding

Structure and Regulation of The Respiratory Syncytial Virus PolymeraseR01GM130950 · NIGMS · EMORY UNIVERSITY · PI Bo Liang · 2019 to 2026
$3.3M
NIGMS NIH HHS R01 GM130950
6 · The paper itself

Abstract

Cryogenic electron microscopy (cryo-EM) has been an important technique for determining the structures of macromolecules at near-atomic resolution in their native states. In recent years, the structures of some respiratory syncytial virus (RSV) protein complexes, such as the RSV polymerase (L:P complex) and nucleoprotein-RNA complex, have been determined by cryo-EM, providing insights into the mechanisms of viral RNA synthesis and facilitating antiviral development. Here, we describe the step-by-step protocols for the negative-stain and cryo-EM analysis of the RSV protein complexes.

Indexed as

Cryoelectron MicroscopyRespiratory Syncytial VirusesRespiratory Syncytial Virus, HumanSingle Molecule ImagingViral ProteinsHumansNegative StainingRNA, ViralRNA, ViralViral ProteinsCryo-freezingCyro-EMEM data processingModel buildingNegative-stain EMNegative stainingRespiratory syncytial virusSingle-particle analysis

Identifiers

PMID40879908

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.