Evidence map›Paper›PMID 40879907›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Expression and Purification of Recombinant Respiratory Syncytial Virus Proteins.

Eliseo Salas, Nicholas Keung, Zhenhang Chen, Yunrong Gao, Dongdong Cao, Bo Liang

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Eliseo SalasDepartment of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA.
Nicholas KeungDepartment of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA.
Zhenhang ChenDepartment of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA.
Yunrong GaoDepartment of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA.
Dongdong CaoDepartment of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA.
Bo LiangDepartment of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA. bo.liang@emory.edu.

Funding

Structure and Regulation of The Respiratory Syncytial Virus PolymeraseR01GM130950 · NIGMS · EMORY UNIVERSITY · PI Bo Liang · 2019 to 2026
$3.3M
NIGMS NIH HHS R01 GM130950
6 · The paper itself

Abstract

Recombinant protein expression and purification are crucial for studying pathogen-host interactions, especially in therapeutic contexts. This chapter comprehensively overviews various expression systems and chromatography techniques to express and purify respiratory syncytial virus (RSV) proteins. These proteins include nonstructural protein 1 (NS1), nonstructural protein 2 (NS2), nucleoprotein (N), phosphoprotein (P), matrix protein (M), M2-1 protein, M2-2 protein, and the large RNA-dependent RNA polymerase protein (L). Choosing an appropriate expression system, such as bacterial E coli cells or insect cells, is critical for protein expression and will depend on the specific size and properties of the particular protein. Chromatography is a common technique in protein purification that leverages the protein's binding affinity, charge, hydrophobicity, or size, but it often requires significant optimization. We outline the protocol for expressing RSV proteins and detail the purification procedures using affinity chromatography, ion exchange chromatography, and size exclusion chromatography to achieve optimal purification of these RSV proteins.

Indexed as

Recombinant ProteinsRespiratory Syncytial VirusesRespiratory Syncytial Virus, HumanViral ProteinsAnimalsChromatography, AffinityChromatography, GelChromatography, Ion ExchangeEscherichia coliGene ExpressionHumansRecombinant ProteinsViral ProteinsAffinity chromatographyE. coli cellsInsect cellsIon exchange chromatographyLMM2–1M2–2NNS1NS2PProtein purificationRecombinant protein expressionRespiratory syncytial virus (RSV)Size exclusion chromatography

Identifiers

PMID40879907

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.