Evidence map›Paper›PMID 40879906›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Secretory Expression and Purification of Respiratory Syncytial Virus G and F Ectodomain in Human Cells.

Ranjini Sankaranarayanan, Binh Ha, Samadhan J Jadhao, Larry J Anderson

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Ranjini SankaranarayananDepartment of Pediatrics, School of Medicine, Emory University, Atlanta, GA, USA.
Binh HaDepartment of Pediatrics, School of Medicine, Emory University, Atlanta, GA, USA.
Samadhan J JadhaoDepartment of Pediatrics, School of Medicine, Emory University, Atlanta, GA, USA.
Larry J AndersonDepartment of Pediatrics, School of Medicine, Emory University, Atlanta, GA, USA. larry.anderson@emory.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Respiratory syncytial virus (RSV) is one of the leading causes of respiratory viral infections in infants, immunocompromised individuals, and the elderly. The important role RSV F and G proteins play in disease progression and their ability to induce neutralizing antibodies make them desirable targets for vaccine production. Access to these expressed and purified proteins is also important to RSV research and diagnostics. Here, we describe methods to produce recombinant, secretory RSV F and G ectodomain in human cells and purify these proteins using Ni-Sepharose affinity chromatography.

Indexed as

Respiratory Syncytial Virus, HumanViral Fusion ProteinsCell LineChromatography, AffinityGene ExpressionHumansProtein DomainsRecombinant ProteinsF protein, human respiratory syncytial virusRecombinant ProteinsViral Fusion ProteinsAffinity chromatographyExpressionF proteinG proteinRespiratory syncytial virusWestern blot

Identifiers

PMID40879906

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.