Evidence map›Paper›PMID 40879794›Full record

ArticleMicrobial ecology2025

Cooperative Interactions Between Bacillus and Lysobacter Enhance Consortium Stability and Fusarium Wilt Suppression in Cucumber.

Xinli Sun, Riyan Xia, Jiyu Xie, Kun Duan, Weibing Xun, Nan Zhang, Guidong Huang, Ruifu Zhang, Qirong Shen, Kai Wu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial ecology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Article
  3. Biofilm synergy byBiofilm · 2026
    Article
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Xinli SunJiangsu Provincial Key Lab for Solid Organic Waste Utilization, Key Lab of Organic-Based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, 210095, China.ORCID http://orcid.org/0000-0003-4969-3058
Riyan XiaJiangsu Provincial Key Lab for Solid Organic Waste Utilization, Key Lab of Organic-Based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, 210095, China.
Jiyu XieJiangsu Provincial Key Lab for Solid Organic Waste Utilization, Key Lab of Organic-Based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, 210095, China.
Kun DuanJiangsu Provincial Key Lab for Solid Organic Waste Utilization, Key Lab of Organic-Based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, 210095, China.
Weibing XunJiangsu Provincial Key Lab for Solid Organic Waste Utilization, Key Lab of Organic-Based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, 210095, China.
Nan ZhangJiangsu Provincial Key Lab for Solid Organic Waste Utilization, Key Lab of Organic-Based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, 210095, China.
Guidong HuangDepartment of Food Science, Foshan University, Foshan, 528231, China.
Ruifu ZhangJiangsu Provincial Key Lab for Solid Organic Waste Utilization, Key Lab of Organic-Based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, 210095, China.
Qirong ShenJiangsu Provincial Key Lab for Solid Organic Waste Utilization, Key Lab of Organic-Based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, 210095, China.
Kai WuYunnan Research Center of Biogas Technology and Engineering, College of Energy and Environment Science, Yunnan Normal University, Kunming, 650500, China. kaiwu8868@hotmail.com.
Zhihui XuJiangsu Provincial Key Lab for Solid Organic Waste Utilization, Key Lab of Organic-Based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, 210095, China. xzh2068@njau.edu.cn.ORCID http://orcid.org/0000-0002-3987-8836

Funding

China Postdoctoral Science Foundation 2023M747140Excellent Postdoctoral Program of Jiangsu Province 2023ZB250National Key Research and Development Program of China 2024YFD1701002National Natural Science Foundation of China 42307173Postdoctoral Fellowship Program of CPSF GZB20230309Rural Revitalization Strategy Project Seed Industry Vitalization Action Project of Guangdong Province 2023WPY00002
6 · The paper itself

Abstract

The rhizosphere microbiome plays a pivotal role in plant health by mediating interactions between hosts, beneficial microbes, and pathogens. However, the ecological mechanisms underlying microbial consortia that suppress soil-borne diseases remain largely unexplored. In this study, we investigated how the biocontrol bacterium Bacillus velezensis SQR9 influences the assembly of the cucumber rhizosphere bacterial community in the presence of the pathogenic fungus Fusarium oxysporum f. sp. cucumerinum (FOC). Inoculation with B. velezensis SQR9 significantly enriched the genus Lysobacter, a known biocontrol taxon. Meta-analysis revealed a positive correlation between Bacillus and Lysobacter abundances in healthy plant rhizospheres-a relationship absent in Fusarium wilt-diseased soils-suggesting a conserved ecological association linked to disease suppression. Mechanistic assays demonstrated that Lysobacter enzymogenes XL8, an antifungal bacterium isolated from the cucumber rhizosphere, formed synergistic biofilms with B. velezensis SQR9. Spent medium growth assays indicated that strain SQR9 facilitated the growth of L. enzymogenes XL8 through metabolic interactions. Targeted RT-qPCR and UHPLC-MS/MS analyses confirmed that treatment with spent medium of the partner strain enhanced the expression and production of antifungal metabolites bacillomycin D and heat-stable antifungal factor (HSAF), both antagonistic to F. oxysporum. Greenhouse trials confirmed that this dual-species consortium more effectively suppressed Fusarium wilt than single-species inoculations, as evidenced by reduced pathogen abundance and enhanced plant growth. Together, our findings underscore the importance of microbial metabolic cooperation and biofilm-mediated coexistence in shaping rhizosphere community assembly and function, providing ecological insights for the development of synthetic microbial consortia aimed at sustainable plant disease management.

Indexed as

BacillusCucumis sativusFusariumLysobacterMicrobial ConsortiaPlant DiseasesBiofilmsMicrobial InteractionsRhizosphereSoil MicrobiologyBiocontrolBiofilmCross-feedingMicrobial interactionSynCom

Identifiers

PMID40879794
PMCPMC12397161

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.