Evidence map›Paper›PMID 40879663›Full record

ArticleJournal of microscopy2025

From cells to pixels: A decision tree for designing bioimage analysis pipelines.

Elnaz Fazeli, Robert Haase, Michael Doube, Kota Miura, David Legland

Abstract read
In one paragraph

Article in Journal of microscopy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Elnaz FazeliBiomedicum Imaging Unit, Faculty of Medicine and HiLIFE, University of Helsinki, Helsinki, Finland.
Robert HaaseData Science Center, Leipzig University, Leipzig, Germany.
Michael DoubeDepartment of Infectious Diseases and Public Health, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China.
Kota MiuraBioimage Analysis & Research, Okayama, Japan.
David LeglandUR BIA, INRAE, Nantes, France.

Funding

Center for Scalable Data Analytics and Artificial Intelligence Dresden/Leipzig
6 · The paper itself

Abstract

Bioimaging has transformed our understanding of biological processes, yet extracting meaningful information from complex datasets remains a challenge, particularly for biologists without computational expertise. This paper proposes a simple general approach, to help identify which image analysis methods could be relevant for a given image dataset. We first categorise structures commonly observed in bioimage data into different types related to image analysis domains. Based on these types, we provide a list of methods adapted to the quantification of images from each category. Our approach includes illustrative examples and a visual flowchart, to help researchers define analysis objectives clearly. By understanding the diversity of bioimage structures and linking them with appropriate analysis approaches, the framework empowers researchers to navigate bioimage datasets more efficiently. It also aims to foster a common language between researchers and analysts, thereby enhancing mutual understanding and facilitating effective communication.

Indexed as

Computational BiologyDecision TreesImage Processing, Computer-Assistedbioimage analysisbiological imagingdecision treeimage processingmicroscopy

Identifiers

PMID40879663
PMCPMC12637018

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.