Evidence map›Paper›PMID 40879486›Full record

ArticleBiochemistry2025

Cysteine Mutagenesis of a Group II Intron-Encoded Protein Supports Splicing, Mobility, and Site-Specific Labeling.

Jasmine A Harper, Sarah A Starcovic, Neil Billington, Aaron R Robart

Abstract read
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In one paragraph

Article in Biochemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jasmine A HarperDepartment of Biochemistry and Molecular Medicine, West Virginia University, Morgantown, West Virginia 26506, United States.
Sarah A StarcovicDepartment of Biochemistry and Molecular Medicine, West Virginia University, Morgantown, West Virginia 26506, United States.
Neil BillingtonDepartment of Biochemistry and Molecular Medicine, West Virginia University, Morgantown, West Virginia 26506, United States.ORCID 0000-0003-2306-0228
Aaron R RobartDepartment of Biochemistry and Molecular Medicine, West Virginia University, Morgantown, West Virginia 26506, United States.ORCID 0000-0002-0518-3972

Funding

Mechanisms of Splicing and RetrotranspositionR01GM133857 · NIGMS · WEST VIRGINIA UNIVERSITY · PI ROBART, AARON REIGH · 2019 to 2023
$1.6M
NIGMS NIH HHS R01 GM133857
6 · The paper itself

Abstract

Group II introns are self-splicing ribozymes that excise themselves from precursor RNA and integrate into new DNA locations through retromobility. Splicing is facilitated by an intron-encoded protein (IEP), a multidomain reverse transcriptase that enhances ribozyme activity and promotes formation of lariat intron-IEP ribonucleoprotein (RNP) complexes. In this study, we examined the role of conserved cysteine residues in the IEP of the group IIC intron

Indexed as

Bacterial ProteinsCysteineIntronsRNA, CatalyticRNA-Directed DNA PolymeraseRNA SplicingThermoanaerobacterBacterial ProteinsCysteineRNA, CatalyticRNA-Directed DNA Polymerase

Identifiers

PMID40879486
PMCPMC12590461

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.