ArticleApplied and environmental microbiology2025
Recovering wastewater RNA for virome sequencing by systematically optimized tangential-flow ultrafiltration and Nanotrap microbiome particles.
Article in Applied and environmental microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Correlation of Norovirus in Wastewater with Gastroenteritis-Related Hospitalizations, New York, USA, 2022-2024.Emerging infectious diseases · 2026Article
- Solvent accessibility of chlorine-reactive amino acid residues in icosahedral virus structures: A meta-analysis.bioRxiv : the preprint server for biology · 2026Article
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9 authors.
Funding
Abstract
Municipal wastewater harbors diverse RNA viruses, which are responsible for many emerging and reemerging diseases in humans, animals, and plants. Although genomic sequencing can be a high-throughput approach for profiling the RNA virome in wastewater, wastewater processing methods often influence sequencing outcomes. Here, we systematically evaluated two wastewater processing methods, tangential-flow ultrafiltration (TFF) and Nanotrap Microbiome A Particles, for detecting the target RNA virus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) via amplicon sequencing and characterizing the RNA virome using whole-transcriptome shotgun sequencing. Our results from paired comparison tests showed that the TFF and Nanotrap methods recovered similar SARS-CoV-2 variants at the lineage level (analysis of similarity [ANOSIM]
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