Evidence map›Paper›PMID 40879186›Full record

ArticleAnnals of neurology2025

Phenotypic Changes in a Monocyte Cluster with High Interleukin-1 Beta Expression during Long-Term Anti-CD20 Therapy.

Mie Waede, Christina Kingo, Karina Damsbo, Maiken Uhrbrand Joergensen, Mhaned Oubounyt, Morten Gjerstorff, Mads Thomassen, Jan Baumbach, Jesper B Moeller, Maria L Elkjaer and 1 more

Abstract read
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In one paragraph

Article in Annals of neurology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Mie WaedeDepartment of Molecular Medicine, University of Southern Denmark, Odense, Denmark.ORCID 0000-0002-7458-9597
Christina KingoDepartment of Molecular Medicine, University of Southern Denmark, Odense, Denmark.
Karina DamsboDepartment of Molecular Medicine, University of Southern Denmark, Odense, Denmark.
Maiken Uhrbrand JoergensenDepartment of Molecular Medicine, University of Southern Denmark, Odense, Denmark.
Mhaned OubounytInstitute for Computational Systems Biology, University of Hamburg, Hamburg, Germany.
Morten GjerstorffCancer Research Unit, University of Southern Denmark, Odense, Denmark.
Mads ThomassenDepartment of Clinical Research, University of Southern Denmark, Odense, Denmark.
Jan BaumbachInstitute for Computational Systems Biology, University of Hamburg, Hamburg, Germany.
Jesper B MoellerDepartment of Molecular Medicine, University of Southern Denmark, Odense, Denmark.
Maria L Elkjaer *Institute for Computational Systems Biology, University of Hamburg, Hamburg, Germany.ORCID 0000-0002-8596-142X
Zsolt Illes *Department of Molecular Medicine, University of Southern Denmark, Odense, Denmark.ORCID 0000-0001-9655-0450

Funding

Danish Regions DanNORMS grantJascha Fonden 2022-0291Novo Nordisk Fonden 0075258Scleroseforeningen A42684
6 · The paper itself

Abstract

objectiveWe aimed to investigate disease-related and anti-CD20 therapy-related changes in peripheral blood mononuclear cells (PBMCs) from multiple sclerosis (MS) patients compared to healthy controls (HC) using multi-omics single-cell analysis.

methodsTargeted single-cell sequencing of transcriptomes and epitopes was performed on PBMCs isolated from 64 blood samples collected from MS patients at baseline and at 3 time points following anti-CD20 treatment, alongside HC. Multicolor spectral flow cytometry was performed on 15 of the samples.

resultsCell cluster analysis identified a subpopulation of classical monocytes with significantly high interleukin-1 beta (IL1B) expression and a pro-inflammatory profile compared to other monocyte clusters. This monocyte cluster expressed genes of pro-inflammatory chemokines and cytokines, such as CXCL8, CCL3, CCL4, and TNFα and was a major cell transmitter subset within the intercellular communication network. The IL1B

interpretationOur single-cell analysis identified a unique peripheral IL1B

Indexed as

Interleukin-1betaMonocytesMultiple SclerosisRituximabAdultAntigens, CD20FemaleHumansLeukocytes, MononuclearMaleMiddle AgedPhenotypeAntigens, CD20IL1B protein, humanInterleukin-1betaRituximab

Identifiers

PMID40879186
PMCPMC12682941

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.