ArticleFEBS letters2025
Development of visible light-sensitive human neuropsin (OPN5) via single amino acid substitution.
Article in FEBS letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Acute Ocular Effects of Violet and Red Light Exposure in Young Adults.Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians (Optometrists) · 2026Article
- A key spectral tuning site of UV-sensitive vertebrate non-visual opsin Opn5.Cellular and molecular life sciences : CMLS · 2025Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Neuropsin (Opn5), a UV-sensitive 'non-visual' opsin, has the potential to be used as optogenetic tools applicable to tissues outside of the eye because of its broad expression. However, its sensitivity to poorly tissue-penetrating UV light poses challenges for its application. In this study, we focused on human OPN5 (hOPN5) to identify amino acid(s) responsible for the UV sensitivity. Sequence alignment across UV-sensitive Opn5s identified a conserved lysine residue (Lys91) at a position implicated in spectral tuning in invertebrate opsins. Substitution of this residue with neutral or acidic amino acids caused substantial shifts in spectral sensitivity towards visible wavelengths. Our findings identify Lys91 as a key spectral tuning site in hOPN5 and provide visible-light-sensitive versions as a candidate for optogenetic applications. Impact statement A "non-visual" opsin, Opn5, is the only UV-sensitive opsin in human. In this study, we identified, for the first time, a key tuning site responsible for the UV sensitivity of hOPN5. In addition to its impact on the opsin molecular studies, this finding could pave the way for the development of novel visual-sensitive Opn5-based optogenetic tools.
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