Evidence map›Paper›PMID 40877762›Full record

ArticleBMC microbiology2025

Epidemiology, antibiotic resistance, and molecular detection of blaOXA and blaCTX-M Genes in ESBL-Producing Escherichia coli from urinary tract infections in Jordanian hospitals.

Ayman Alsheikh, Abdalla Abuawwad, Israa Yousef, Dhia'a Abueswailem, Hafez Al-Momani, Walid Aburayyan, Nesrin Seder, Montasir Al-Mansi, Mohammad Al-Najjar, Lana Al-Shoubaki and 2 more

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Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Ayman AlsheikhDepartment of Medical Laboratory Science, Faculty of Allied Medical Sciences Zarqa University, P.O. Box 2000, Zarqa, 13110, Jordan. ayman.alsheikh74@gmail.com.ORCID 0000-0002-1151-1178
Abdalla AbuawwadDepartment of Medical Laboratory Science, Faculty of Allied Medical Sciences Zarqa University, P.O. Box 2000, Zarqa, 13110, Jordan.ORCID 0000-0003-4518-5873
Israa YousefDepartment of Medical Laboratory Science, Faculty of Allied Medical Sciences Zarqa University, P.O. Box 2000, Zarqa, 13110, Jordan.ORCID 0000-0002-0538-8900
Dhia'a AbueswailemDepartment of Applied Speech Language Pathology and Audiology, Faculty of Allied Medical Sciences, Zarqa University, P.O. Box 2000, Zarqa, 13110, Jordan.ORCID 0000-0001-6522-3975
Hafez Al-MomaniDepartment of Microbiology, Pathology and Forensic Medicine, Faculty of Medicine, The Hashemite University, P. O Box 330127, Zarqa, 13133, Jordan.ORCID 0000-0003-2119-0811
Walid AburayyanDepartment of Medical Laboratory Analysis, Faculty of Science, Al-Balqa Applied University, P.O Box 8615, Amman, 11121, Jordan.ORCID 0009-0006-2447-654X
Nesrin SederDepartment of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmacy, Applied Science Private University, P.O.Box 541350, Amman, 11937, Jordan.ORCID 0000-0001-9064-9257
Montasir Al-MansiDepartment of Medical Sciences, Aqaba Medical Sciences University (AMSU), P.O box 77110, Aqaba, Jordan.ORCID 0000-0001-8840-8366
Mohammad Al-NajjarFaculty of Pharmacy, Applied Science Private University, Amman, Jordan.ORCID 0000-0003-0001-6902
Lana Al-ShoubakiDepartment of Medical Laboratory Science, Faculty of Allied Medical Sciences Zarqa University, P.O. Box 2000, Zarqa, 13110, Jordan.ORCID 0000-0001-5794-0325
Yaqeen RjoubDepartment of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Al-Albayt University, Mafraq, 25113, Jordan.ORCID 0009-0006-2386-9392
Nawal Al-Zaa'qDepartment of Medical Laboratory Science, Faculty of Allied Medical Sciences Zarqa University, P.O. Box 2000, Zarqa, 13110, Jordan.ORCID 0009-0009-1541-1244

Funding

Zarqa University 13659
6 · The paper itself

Abstract

backgroundUrinary tract infections (UTIs) impose significant challenges among patients infected with multidrug-resistant Escherichia coli (E. coli). The emergence of the extended-spectrum beta-lactamase (ESBL) strains complicates the treatment and exacerbates life-threatening infections. The comprehensive analysis of the resistance mechanisms combined with identifying the relevant genes amend the implications encountered with the devastating effects generated by the pathogenic microorganisms. This study sought about the prevalence of ESBL-producing E. coli in UTIs patients in Jordan, focusing on the prevalence of blaOXA and blaCTX-M resistance genes.

methodsA total of 500 urine samples were compiled from patients diagnosed with UTIs between November 2023 and May 2024. The samples were collected from five governmental hospitals distributed in three districts in Jordan (North, Middle, and South). Urine samples were cultured in the microbiology lab and the percentage of E. coli isolates was explored. Later on, isolates were tested for ESBL production using the double disk synergy test. Thus, molecular detection of the blaOXA and blaCTX-M genes was performed using polymerase chain reaction (PCR) assays. Statistical analysis was conducted to assess the prognostic correlation between the prevalence of resistance genes and variables such as location, age, and gender.

resultsIn the current study, we investigated the prevalence of ESBL-producing E. coli in UTIs across Jordanian hospitals, focusing on the prevalence of the blaOXA and blaCTX-M genes with the correlation with the epidemiological factors. Our findings revealed an overall prevalence of 18% for ESBL- E. coli isolates, with the highest prevalence in the Northern governorates exhibiting 50% (OR = 1.67, 95% CI: 1.103-2.519). Females showed a higher prevalence of the blaOXA gene (62.29%) compared to males (37.7%) (p = 0.008). Moreover, the blaCTX-M gene was universally present in all samples with a prevalence of 98%. The multinomial regression analysis has revealed a significant correlation (p = 0.001) between location on the distribution of the blaOXA gene among the study population.

conclusionThe findings of the current study highlight alarming rates for the prevalence of ESBL-producing E. coli in UTIs across Jordan in general and in the northern districts as specific. Emphasizing the crucial need for effective surveillance, antibiotic stewardship, and infection control strategies. The significant regional variations in resistance rates suggest that localized factors, such as antibiotic misuse and environmental influences combined with the widespread of blaCTX-M and blaOXA genes point to the need for targeted antibiotic resistance surveillance in Jordan.

Indexed as

beta-LactamasesDrug Resistance, Multiple, BacterialEscherichia coliEscherichia coli InfectionsEscherichia coli ProteinsUrinary Tract InfectionsAdolescentAdultAgedAged, 80 and overAnti-Bacterial AgentsChildChild, PreschoolFemaleHospitalsHumansAnti-Bacterial Agentsbeta-LactamasesEscherichia coli ProteinsEscherichia Coli (E. Coli)Extended Spectrum β-Lactamase (ESBL)JordanUrinary Tract Infection (UTI)

Identifiers

PMID40877762
PMCPMC12392507

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