ArticleMethods in molecular biology (Clifton, N.J.)2025
Chemical Strategies to Enhance Antisense Strand Selection and Minimize Off-Target Effect-Mediated by siRNAs.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Abstract
Small interfering RNAs (siRNAs) hold significant promise as therapeutic agents for treating human diseases. However, their use is often limited by unintended off-target effects arising from both the sense and antisense strands. Since the 5'-phosphate group on either strand is necessary for recognition by Argonaute 2 (AGO2), the core protein in the RNA-induced silencing complex (RISC), blocking phosphorylation of the sense strand may prevent its incorporation into RISC, thereby reducing potential off-target effects. In this chapter, we describe the use of biotin, a naturally occurring compound, to block 5'-end phosphorylation on either the sense or antisense strand to prevent their selection by AGO2. This strategy should offer a practical option to enhance siRNA specificity and efficacy.
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