ArticleMethods in molecular biology (Clifton, N.J.)2025
Optimization of In Vitro Transcription Reaction for mRNA Production Using Chromatographic At-Line Monitoring.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- High-throughput monitoring of free MgAnalytical and bioanalytical chemistry · 2026Article
Corrections and comments
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Authors and funding
5 authors.
Funding
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Abstract
mRNA synthesis by in vitro transcription reaction (IVT) is a critical step in the production of mRNA therapeutics. IVT is a complex, multi-component enzymatic reaction where mRNA is synthesized from a DNA template using RNA polymerase, typically T7 RNA polymerase, and nucleoside triphosphates (NTPs) in a buffered solution containing magnesium ions. The concentrations and ratios between reaction components need to be carefully balanced to maximize mRNA yield while minimizing the formation of product-related impurities and the cost of the reaction. This protocol describes the use of a chromatographic analytical method for separation of NTPs, pDNA, and mRNA with a 4-minute read-out. At-line analysis which allows to monitor mRNA production and NTP depletion can be used to study the influence of reaction parameters on reaction kinetics and yield as well as for reaction optimization and transfer to fed-batch mode.
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Registered trials
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