Evidence map›Paper›PMID 40877505›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Hydrophobic Chromatography Purification of Linearized Plasmid DNA Reduces dsRNA Formation During In Vitro Transcription.

Juan Martínez-Oliván, Verónica Lampaya, Ana Larraga, Héctor Magallón, Julia Domínguez, Víctor Navarro, Diego Casabona

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Juan Martínez-OlivánCertest Pharma, RNA Synthesis and Development Department, Certest Biotec, Zaragoza, Spain.
Verónica LampayaCertest Pharma, RNA Synthesis and Development Department, Certest Biotec, Zaragoza, Spain.
Ana LarragaCertest Pharma, RNA Synthesis and Development Department, Certest Biotec, Zaragoza, Spain.
Héctor MagallónCertest Pharma, RNA Synthesis and Development Department, Certest Biotec, Zaragoza, Spain.
Julia DomínguezCertest Pharma, RNA Synthesis and Development Department, Certest Biotec, Zaragoza, Spain.
Víctor NavarroCertest Pharma, RNA Synthesis and Development Department, Certest Biotec, Zaragoza, Spain.
Diego CasabonaCertest Pharma, RNA Synthesis and Development Department, Certest Biotec, Zaragoza, Spain. dcasabona@certest.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Double-stranded RNA (dsRNA) formation during in vitro transcription is a significant challenge due to its potent immunogenic properties. In this chapter, we present an optimized chromatographic method for purifying linearized DNA templates, effectively reducing dsRNA impurities during mRNA production. The findings highlight the critical role of template quality, demonstrating how template impurities directly contribute to dsRNA formation. Moreover, we show that additional polishing purification steps further decrease dsRNA levels, reducing the activation of innate immune responses mediated by dsRNA-sensing receptors such as Toll-like receptor 3.

Indexed as

DNAPlasmidsRNA, Double-StrandedTranscription, GeneticHumansHydrophobic and Hydrophilic InteractionsDNARNA, Double-StrandedAffinity chromatographydsRNAHydrophobic chromatographyImmunogenicityIVTmRNAVaccines

Identifiers

PMID40877505

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.