Evidence map›Paper›PMID 40877504›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Lateral Flow Immunoassay for Rapid and Sensitive Detection of dsRNA Contaminants in In Vitro-Transcribed mRNA Products.

Dengwang Luo, Daming Wang, Yuhong Cao

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Dengwang LuoState Key Laboratory of Synthetic Biology, Frontiers Science Center for Synthetic Biology (Ministry of Education), School of Synthetic Biology and Biomanufacturing, Tianjin University, Tianjin, China.
Daming WangBiosynRNA Biotechnology Company, Beijing, China.
Yuhong CaoCAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Chinese Academy of Sciences, Beijing, China. caoyh@nanoctr.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Double-stranded RNA (dsRNA), often generated as a by-product during in vitro transcription, poses a significant challenge to therapeutic applications of mRNA. In this chapter, we present a rapid and sensitive lateral flow strip assay (LFSA) utilizing colloidal gold nanoparticles for detecting dsRNA contaminants in in vitro-transcribed mRNA. The assay delivers results within 15 min and achieves a limit of detection (LOD) of 69.32 ng/mL for N1-methyl-pseudouridine-containing dsRNA. This method provides a quick and efficient tool for ensuring mRNA purity, reducing the risk of dsRNA-induced immunogenicity, and facilitating large-scale mRNA production without the need for time-consuming traditional methods.

Indexed as

RNA, Double-StrandedRNA, MessengerGoldGold ColloidImmunoassayLimit of DetectionMetal NanoparticlesGoldGold ColloidRNA, Double-StrandedRNA, MessengerDouble-stranded RNAImmunogenicityIn vitro-transcribed mRNALateral flow strip assaymRNA purityRapid detection

Identifiers

PMID40877504

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.