ArticleScientific data2025
A Comprehensive Mass Spectrometry-Based Proteomic Dataset of Human Spermatozoa.
Article in Scientific data, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
2 citing papers in PubMed.
- Prostatitis-Related Male Infertility: From Inflammation and Dysbiosis to Sperm DNA Damage.Diagnostics (Basel, Switzerland) · 2026Review
- A Comprehensive Mass Spectrometry-Based Proteomic Dataset of Human Spermatozoa.Scientific data · 2025Article
Corrections and comments
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Authors and funding
4 authors.
Funding
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Abstract
Male infertility is fundamentally rooted in developmental defects of germ cells and associated molecular dysregulation, yet the underlying mechanisms remain poorly understood. Data-independent acquisition (DIA) has emerged as a powerful tool in discovery proteomics, enabling the identification of disease biomarkers, therapeutic targets, and molecular pathways with high precision and reproducibility. To analyse the aberrant regulatory events in human sperm proteins associated with male reproductive disorders in a high-throughput, reproducible, and reliable manner, we employed the Orbitrap Astral mass spectrometer, which independently operates Orbitrap Full Scan and Astral MS/MS to generate high-resolution full-scan spectra and high-quality secondary maps. Leveraging the principles of DIA technology, we constructed the most comprehensive human sperm proteomic expression profile reported to date, encompassing 9,309 proteins, 198,153 unique precursors, 154,062 modified peptides, and 145,355 peptides. Moreover, this data is derived from two types of sperm samples to facilitate the exploration of disease-specific proteins. Our data serve as a valuable resource for analysing spermatozoa protein content, facilitating deeper insights into male reproduction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.