ArticlePloS one2025
Single nucleus RNA sequencing of the cerebral cortex from genetically diverse inbred mouse strains reveals differences in pericyte and endothelial cell composition.
Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Endothelial Cell-Pericyte Crosstalk in the Brain Balances Microvascular Stability and Plasticity.Arteriosclerosis, thrombosis, and vascular biology · 2026Review
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4 authors.
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Abstract
Genetic background influences animal behavior and susceptibility to brain diseases. To evaluate how genetic background influences the relative number and/or types of cells in the brain, we performed single nucleus RNA sequencing (snRNAseq) on fourteen genetically distinct collaborative cross (CC) inbred mouse strains. These data comprise over 287,000 nuclei derived from 92 samples across the strains. We identified 12 principal cell types and 60 refined cell types in each of the strains. Pericytes and endothelial cells were the two principal cell types to show a statistically significant difference in cell proportion between strains. We validated these findings histologically by staining for pericyte (CD13) and endothelial cell (PECAM-1) markers. Consistent with our snRNAseq analyses, we histologically observed differences in pericyte and endothelial cell counts between strains. In addition, we found that the proportion of certain subsets of excitatory and inhibitory neurons varied in some strains. Overall, our study suggests that genetic background can influence brain cell type composition, with a notable influence on cells that make up the neurovascular unit.
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