ArticleMethods in molecular biology (Clifton, N.J.)2025
Synthesizing Defined Ubiquitin-Modified SUMO Dimers.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Ubiquitination and SUMOylation interactions between proteins play a critical role in various biological processes and the progression of diseases. To investigate the biological functions of the direct crosstalk between protein ubiquitination and SUMOylation biochemically, we have developed a method for synthesizing Ub-tagged SUMO2 dimers using an expanding genetic code approach. In this description, we outline the procedures for creating Ub-SUMO heterodimers through the incorporation of defined noncanonical amino acids (ncAAs) and biorthogonal functional group-guided conjugation techniques. These homogeneous Ub-SUMO heterodimers will serve as valuable tools for studying the mechanisms of Ub-SUMO chain elongation, degradation, topology, and other related protein-protein interactions.
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