Evidence map›Paper›PMID 40875114›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

A Fluorometric Assay to Compare SUMO Isoform Preferences in the SENP/ULP Family.

Nathalia Varejão, Lucía Sánchez-Alba, David Reverter

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Nathalia VarejãoInstitut de Biotecnologia i de Biomedicina (IBB) and Dept. de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Spain. nathalia.varejao@uab.cat.
Lucía Sánchez-AlbaInstitut de Biotecnologia i de Biomedicina (IBB) and Dept. de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Spain.
David ReverterInstitut de Biotecnologia i de Biomedicina (IBB) and Dept. de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Spain. david.reverter@uab.cat.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Comparison of proteolytic activities of deconjugating enzymes toward ubiquitin or other ubiquitin-like modifiers (UbL), such as SUMO isoforms or Nedd8, has been a central focus in recent years to understand the dual or even multiple activities exhibited by members of the SENP/ULP family. This protocol outlines a competitive assay for an initial kinetic assessment of a SENP/ULP family member with specificity for alternative SUMO substrates. By using this method, the affinity of a SENP/ULP member for different SUMO isoforms or SUMO mutant variants can be compared. Also, IC

Indexed as

Cysteine EndopeptidasesEndopeptidasesFluorometrySmall Ubiquitin-Related Modifier ProteinsHumansKineticsNEDD8 ProteinProtein IsoformsSubstrate SpecificityCysteine EndopeptidasesEndopeptidasesNEDD8 ProteinProtein IsoformsSmall Ubiquitin-Related Modifier ProteinsDeconjugationDUBsIC50IsopeptidaseSENPSUMOUbiquitinULP

Identifiers

PMID40875114

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.