Evidence map›Paper›PMID 40875101›Full record

ArticleBulletin of experimental biology and medicine2025

Ex Vivo Irradiation Protocol in Murine Lymph Node Cells.

M N Yurova, E I Fedoros, A B Danilova, T L Nekhaeva, N V Emelyanova, A V Grigorevskaya, I G Popovich, I A Baldueva

Abstract read
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In one paragraph

Article in Bulletin of experimental biology and medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

M N YurovaN. N. Petrov National Medical Research Center of Oncology, Ministry of Health of Russian Federation, St. Petersburg, Russia. yumarni@gmail.com.
E I FedorosN. N. Petrov National Medical Research Center of Oncology, Ministry of Health of Russian Federation, St. Petersburg, Russia.
A B DanilovaN. N. Petrov National Medical Research Center of Oncology, Ministry of Health of Russian Federation, St. Petersburg, Russia.
T L NekhaevaN. N. Petrov National Medical Research Center of Oncology, Ministry of Health of Russian Federation, St. Petersburg, Russia.
N V EmelyanovaN. N. Petrov National Medical Research Center of Oncology, Ministry of Health of Russian Federation, St. Petersburg, Russia.
A V GrigorevskayaN. N. Petrov National Medical Research Center of Oncology, Ministry of Health of Russian Federation, St. Petersburg, Russia.
I G PopovichN. N. Petrov National Medical Research Center of Oncology, Ministry of Health of Russian Federation, St. Petersburg, Russia.
I A BalduevaN. N. Petrov National Medical Research Center of Oncology, Ministry of Health of Russian Federation, St. Petersburg, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim was to test the protocol for obtaining lymphoid tissue from mouse lymph nodes and characterize the level of DNA damage with analysis of the repair and apoptosis protein markers in ex vivo model of cell irradiation. The lymph node cell suspension was exposed to X-ray irradiation at a dose of 2 Gy and compared with a sham-irradiated (0 Gy) control. Ex vivo irradiation at a dose of 2 Gy after 3 h caused a 7-fold increase in DNA damage level determined in DNA comet assay. A significant increase in the level of repair proteins γH2AX (by 3.7 times), Rad51 (by 2.2 times), and XPA (by 28 times), cell cycle arrest proteins Cdc25A (by 2.6 times), p21 (by 10 times), and p16 (by 5 times), and pro-apoptotic protein Bax (by 3.8 times) was noted, without significant changes in Bcl-2 level. The proposed technique allows quick obtaining lymphoid tissue samples suitable for analysis of the radiosensitivity of lymphoid cells in mice of various strains, changes in their sensitivity after or in combination with the primary damaging exposure.

Indexed as

DNA DamageLymph NodesAnimalsApoptosisbcl-2-Associated X ProteinComet AssayDNA RepairHistonesLymphocytesMaleMiceRad51 RecombinaseRadiation ToleranceX-Raysbcl-2-Associated X ProteinHistonesRad51 Recombinaseapoptosisex vivo irradiationlymph nodeoncologyrepair

Identifiers

PMID40875101

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.