ArticleNucleic acids research2025
A DNA nicking Class 1 OLD family nuclease mediates phage defense in Vibrio cholerae and is countered by a phage-encoded inhibitor.
Article in Nucleic acids research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed.
- Repurposing anti-phage defenses to differentially arrest the viral lifecycle reveals the regulatory logic of a parasitic satellite.mSystems · 2026Article
- Repurposing anti-phage defenses to differentially arrest the viral lifecycle reveals the regulatory logic of a parasitic satellite.bioRxiv : the preprint server for biology · 2026Article
- It's not me, it's you: Anti-phage nuclease specificity inside a bacterium.PLoS pathogens · 2026Article
- Bacteriophage deploys a RecA-dependent nuclease to inhibit Staphylococcus aureus replication and promote phage propagation.Nucleic acids research · 2026Article
- The uncharacterizedmicroPublication biology · 2026Article
- Specificity and mechanism of tRNA cleavage by the AriB Toprim nuclease of the PARIS bacterial immune system.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Article
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Abstract
Bacteria are constantly threatened by their viral predators (phages), which has resulted in the development of defense systems for bacterial survival. One family of defense systems found widely across bacteria are OLD (for overcoming lysogenization defect) family nucleases. Despite recent discoveries regarding Class 2 and 4 OLD family nucleases and how phages overcome them, Class 1 OLD family nucleases remain underexplored, with only one antiphage Class 1 OLD family nuclease described to date. Here, we identify the first bacterially encoded Class 1 OLD family nuclease with antiphage activity: the Vibrio cholerae-encoded Vc OLD. We describe its disruption of genome replication of the lytic vibriophage ICP1. Furthermore, we examine its in vitro activity, identifying Vc OLD as a DNA nickase. Finally, we identify the first direct inhibitor of a Class 1 OLD family nuclease, the ICP1-encoded Oad1. Our research sheds new light on the role of Class 1 OLD family nucleases in phage defense and highlights the dynamic arms race between V. cholerae and its predatory phage ICP1.
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