ArticleFrontiers in cell and developmental biology2025
Chloroquine mitigates long-term effects of in vitro culture in mouse embryos.
Article in Frontiers in cell and developmental biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
3 citing papers in PubMed.
- Preimplantation culture conditions and later-life outcomes: implications for evaluating embryo culture media.The Journal of reproduction and development · 2026Review
- Excess glutamine in embryo culture media alters mitochondrial and autophagy-related dynamics associated with impaired postnatal development.Frontiers in cell and developmental biology · 2026Article
- How Early-Life Programming During Embryogenesis Imprints Cellular Memory.International journal of molecular sciences · 2025Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Methods: Two-cell stage embryos were obtained by mating ICR female mice with ICR male mice, followed by oviduct flushing. The recovered embryos were cultured Results: We found that low concentration CQ significantly reduced developmental rate and total cell count in a CQ concentration-dependent manner (control: 67 ± 2.5 vs. 48 ± 2.3 with 1.0 µM CQ vs. 37 ± 2.9 with 2.0 µM CQ), as well as the numbers of trophectoderm (TE) and inner cell mass (ICM) cells. These results suggest that low concentration CQ treatment may suppress cell proliferation likely by inhibiting nutrient supply via autophagy. Notably, after implantation, the 2.0 µM CQ-treated group exhibited increased pups rate and reduced body weight comparable to the naturally mated group, and glucose tolerance similar to that of the naturally mated group, in contrasted to the untreated group. Discussion: These findings suggest that inhibiting autophagy during preimplantation development may mitigate the long-term effects of
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Registered trials
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