ArticleISME communications2025
Quantitative metagenomics for marine prokaryotes and photosynthetic eukaryotes.
Article in ISME communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- The dynamic mesoscale sink and source niches for eukaryotic phytoplankton in a subtropical gyre.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Accu16S/AccuITS: Accurate and broadly applicable amplicon sequencing for absolute microbiome quantification.iMeta · 2026Article
- Converting relative amplicon abundances to absolute abundances via flow cytometry: metagenomic validation and application to long ocean transects.ISME communications · 2026Article
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Authors and funding
10 authors.
Funding
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Abstract
High-throughput sequencing has provided unprecedented insights into microbial biodiversity in marine and other ecosystems. However, most sequencing-based studies report only relative (compositional) rather than absolute abundance, limiting their application in ecological modeling and biogeochemical analyses. Here, we present a metagenomic protocol incorporating genomic internal standards to quantify the absolute abundances of prokaryotes and eukaryotic phytoplankton, which together form the base of the marine food web, in unfractionated seawater. We applied this method to surface waters collected across 50°N to 40°S during the 29
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