Evidence map›Paper›PMID 40873471›Full record

ArticleBio-protocol2025

Production of Homogeneous, Functional Zinc-Finger Arrays in High Yield With Two Chromatographic Steps.

Jingchang Liang, Maia Azubel, Guanqiao Wang, Yan Nie, Roger D Kornberg, Andrew J Beel, Pierre-Jean Matteï

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jingchang LiangDepartment of Structural Biology, Stanford University, Stanford, CA, USA.
Maia AzubelDepartment of Structural Biology, Stanford University, Stanford, CA, USA.
Guanqiao WangShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China.
Yan NieShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China.
Roger D KornbergDepartment of Structural Biology, Stanford University, Stanford, CA, USA.
Andrew J BeelDepartment of Structural Biology, Stanford University, Stanford, CA, USA.
Pierre-Jean MatteïDepartment of Structural Biology, Stanford University, Stanford, CA, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Zinc-finger (ZF) arrays are compact, sequence-specific polynucleotide-binding domains, which have been used to target the delivery of diverse effector domains, enabling applications such as gene identification, localization, regulation, and editing. To facilitate in vitro applications of ZF arrays, we have developed a general method for their expression and purification. Here, we describe a protocol involving two chromatographic steps that yields homogeneous and functional ZF arrays in milligram quantities. Key features • A general method for expressing and purifying C2H2 ZF arrays in

Indexed as

Cation exchange chromatographyHis-SUMO tagNi-NTA chromatographyOn-column cleavageProtein purificationZinc fingers

Identifiers

PMID40873471
PMCPMC12378415

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.