ArticleJournal of veterinary internal medicine
Effect of Long-Term Freezing on Indirect Fluorescent Antibody Titers for the Diagnosis of Equine Protozoal Myeloencephalitis.
Article in Journal of veterinary internal medicine. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Seroprevalence of Sarcocystis neurona and Neospora hughesi in healthy horses from the province of Alberta, Canada.Journal of veterinary internal medicine · 2026Article
- Effect of Long-Term Freezing on Indirect Fluorescent Antibody Titers for the Diagnosis of Equine Protozoal Myeloencephalitis.Journal of veterinary internal medicineArticle
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
backgroundLong-term freezing storage can alter the stability of proteins, thereby compromising accurate determination of indirect fluorescent antibody test (IFAT) titers that support the diagnosis of equine protozoal myeloencephalitis.
objectiveAssess the effect of long-term storage at -80°C on IFAT against S. neurona and N. hughesi in equine serum and cerebrospinal fluid (CSF). SAMPLES: Paired serum and CSF (n = 46), and serum only (n = 25) samples.
methodsProspective study of samples stored 6-12, 13-18, and 19-24 months. Comparing antibody titers across time points, McNemar and Wilcoxon rank tests were used for statistical analysis.
resultsAfter long-term freezing storage for 19-24 months, N. hughesi IFAT results for CSF were significantly different compared with their fresh-testing time point (p = 0.04), but a similar difference was not observed for serum samples. No statistical differences were observed for serum or CSF samples tested for S. neurona after 6-12, 13-18, or 19-24 months compared with their original fresh testing results. However, the serum to CSF ratio cutoff of ≤ 64 in support of disease changed in 41% (19 of 46) of paired samples. CONCLUSIONS AND CLINICAL IMPORTANCE: Freezing samples at -80°C for more than 18 months might alter test results, leading to possible changes in serum to CSF ratio calculations and affecting the interpretation of results for clinical and research purposes.
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