Evidence map›Paper›PMID 40873000›Full record

ArticleMolecular reproduction and development2025

Characterization of the Lipid Profile of Immature and In Vitro Matured Cat Oocytes.

Erlandia M Vasconcelos, Thais G de Oliveira, Ribrio I T P Batista, Paulo S C Rangel, Georgia C Atella, Gabriela R Leal, Joanna M G Souza-Fabjan

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Article in Molecular reproduction and development, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Erlandia M VasconcelosFaculdade de Veterinária, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
Thais G de OliveiraFaculdade de Veterinária, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
Ribrio I T P BatistaFaculdade de Veterinária, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
Paulo S C RangelFaculdade de Veterinária, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
Georgia C AtellaLaboratório de Bioquímica de Lipídios e Lipoproteínas, Instituto de Bioquímica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
Gabriela R LealFaculdade de Veterinária, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
Joanna M G Souza-FabjanFaculdade de Veterinária, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.

Funding

This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico, Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
6 · The paper itself

Abstract

Forskolin (FSK), l-carnitine (LC), and Conjugated Linoleic Acid (CLA) are lipid modulators that reduce cellular lipid content. This study characterizes the lipid profile and mRNA content of immature and in vitro matured cat oocytes with or without a MIX of these modulators. Ovaries were collected, COC retrieved, and allocated into three groups: immature (IM), matured without (CONT), or with FSK, LC, and CLA (MIX). Lipid analysis was performed using High-performance thin-layer chromatography, gene expression by RT-qPCR, and oocytes stained with Nile Red, Oil Red O, and Sudan Black B. Eight lipid subclasses were detected in all groups. Esterified sterol was more abundant in CONT than IM, with intermediate levels in MIX. Triacylglycerol was higher in MIX than IM, while monoacylglycerol increased in both CONT and MIX. In MIX, DGAT1, FABP3, and PLIN2 were upregulated compared to IM. Moreover, in IM, DGAT1 was downregulated and FABP3 upregulated relative to CONT. FABP3 levels were higher in MIX than CONT. Oil Red O and Sudan Black B staining showed reduced lipid content in MIX compared to CONT, while Nile Red detected no difference. In conclusion, feline oocytes subjected to MIX during in vitro maturation exhibited increased triacylglycerol levels and enhanced FABP3 expression.

Indexed as

In Vitro Oocyte Maturation TechniquesLipid MetabolismLipidsOocytesAnimalsCatsFemaleLipidschemical delipidationfeline oocytelipid metabolismlipid modulators

Identifiers

PMID40873000
PMCPMC12391639

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.