Evidence map›Paper›PMID 40871365›Full record

ArticleMicroorganisms2025

Variabilities in N2 and E Gene Concentrations in a SARS-CoV-2 Wastewater Multiplex Assay.

Ashley Green, Aiswarya Rani Pappu, Melanie Oakes, Suzanne Sandmeyer, Matthew Hileman, Sunny Jiang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microorganisms, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ashley GreenDepartment of Civil and Environmental Engineering, University of California Irvine, Irvine, CA 92697, USA.ORCID 0009-0000-3524-2817
Aiswarya Rani PappuDepartment of Civil and Environmental Engineering, University of California Irvine, Irvine, CA 92697, USA.
Melanie OakesDepartment of Biological Chemistry, University of California Irvine, Irvine, CA 92697, USA.ORCID 0009-0002-2369-4810
Suzanne SandmeyerDepartment of Biological Chemistry, University of California Irvine, Irvine, CA 92697, USA.
Matthew HilemanJoe C. Went School of Population and Public Health, University of California Irvine, Irvine, CA 92697, USA.
Sunny JiangDepartment of Civil and Environmental Engineering, University of California Irvine, Irvine, CA 92697, USA.ORCID 0000-0002-4993-8038

Funding

EPA 84025701UCI COVID CARFT n/aU.S. National Science Foundation 2027306Water Research Foundation 5093
6 · The paper itself

Abstract

Wastewater can serve as both a source of pathogens that pose risks to human health and a valuable resource for tracking and predicting disease prevalence through wastewater-based surveillance (WBS). In WBS for SARS-CoV-2, both nucleocapsid-specific (N1 and N2) and the envelope (E) genes are common targets for primer design, but ambiguity remains regarding differences in results depending on the gene target chosen. This study investigated how and why two SARS-CoV-2 gene targets (N2 and E) varied when analyzed in a multiplex RT-ddPCR assay for a COVID-19 wastewater monitoring study. From December 2021 to June 2022, over 700 raw wastewater samples were collected from thirteen manholes in the University of California, Irvine sewer system. Murine hepatitis virus (MHV) was used as a matrix recovery and process control in the triplex RT-ddPCR assay. Water quality tests (TSS, COD, pH, turbidity and NH

Indexed as

COVID-19manholeprimerrecovery efficiencyRT-ddPCRwastewater-based surveillance

Identifiers

PMID40871365
PMCPMC12388642

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.