Evidence map›Paper›PMID 40871360›Full record

ArticleMicroorganisms2025

Swift Realisation of Wastewater-Based SARS-CoV-2 Surveillance for Aircraft and Airports: Challenges from Sampling to Variant Detection.

Cristina J Saravia, Kira Zachmann, Natalie Marquar, Ulrike Braun, Claus Gerhard Bannick, Timo Greiner, Peter Pütz, Susanne Lackner, Shelesh Agrawal

Abstract read
In one paragraph

Article in Microorganisms, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Cristina J SaraviaWastewater Treatment Technology, Wastewater Disposal, German Environment Agency, 14195 Berlin, Germany.ORCID 0009-0006-7646-7215
Kira ZachmannChair of Water and Environmental Biotechnology, Institute IWAR, Department of Civil and Environmental Engineering Sciences, Technical University Darmstadt, 64287 Darmstadt, Germany.ORCID 0009-0006-0674-7991
Natalie MarquarWastewater Analysis, Monitoring Methods, German Environment Agency, 12307 Berlin, Germany.
Ulrike BraunWastewater Analysis, Monitoring Methods, German Environment Agency, 12307 Berlin, Germany.ORCID 0000-0002-3682-3637
Claus Gerhard BannickWastewater Treatment Technology, Wastewater Disposal, German Environment Agency, 14195 Berlin, Germany.
Timo GreinerDepartment of Infectious Disease Epidemiology, Robert Koch Institute, 13353 Berlin, Germany.
Peter PützDepartment of Infectious Disease Epidemiology, Robert Koch Institute, 13353 Berlin, Germany.ORCID 0000-0002-2948-0189
Susanne LacknerChair of Water and Environmental Biotechnology, Institute IWAR, Department of Civil and Environmental Engineering Sciences, Technical University Darmstadt, 64287 Darmstadt, Germany.
Shelesh AgrawalChair of Water and Environmental Biotechnology, Institute IWAR, Department of Civil and Environmental Engineering Sciences, Technical University Darmstadt, 64287 Darmstadt, Germany.ORCID 0000-0001-9365-5951

Funding

Federal Ministry of Health AMELAG
6 · The paper itself

Abstract

International air traffic has contributed to the global spread of SARS-CoV-2 and its variants. In early 2023, wastewater-based epidemiology (WBE) has been implemented at airports as a surveillance tool to detect emerging variants at short notice. This study investigates the feasibility and challenges of applying WBE at Berlin Brandenburg (BER) Airport, including a rapid implementation of wastewater sampling and analysis under unprecedented circumstances. For this purpose, aircraft and airport wastewater was sampled over 13 weeks. Established sampling and analysis protocols for municipal wastewater treatment plants (WWTPs) had to be adapted to the specific conditions of the airport environment. SARS-CoV-2 RNA was quantified and sequenced, revealing SARS-CoV-2 mutations not previously observed in clinical surveillance data in Germany. Despite the logistical and methodological challenges, the study demonstrates that WBE can serve as an early warning system for pathogen introduction. However, our study also underscores the need for realistic timelines for the establishment and validation of WBE monitoring strategies in new contexts. Investments in the establishment of WBE systems, e.g., infrastructure, protocols, trained personnel, and a network of stakeholders at strategic nodes including airports, can act as an effective tool for pandemic preparedness and global health security.

Indexed as

aircraftairportdigital PCRnext-generation sequencingSARS-CoV-2wastewater sampling methodswastewater surveillance

Identifiers

PMID40871360
PMCPMC12388640

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.