Evidence map›Paper›PMID 40871225›Full record

ArticleMicroorganisms2025

Quantitative Evaluation of Endogenous Reference Genes for RT-qPCR and ddPCR Gene Expression Under Polyextreme Conditions Using Anaerobic Halophilic Alkalithermophile

Xinyi Tao, Qinghua Xing, Yingjie Zhang, Belsti Atnkut, Haozhuo Wei, Silva Ramirez, Xinwei Mao, Baisuo Zhao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microorganisms, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xinyi TaoGraduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Qinghua XingGraduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China.ORCID 0009-0000-2343-4811
Yingjie ZhangGraduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Belsti AtnkutDepartment of Biology, College of Natural and Computational Science, Injibara University, Injibara P.O. Box 40, Ethiopia.ORCID 0000-0002-8441-6833
Haozhuo WeiGraduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Silva RamirezGraduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China.ORCID 0009-0006-7929-5789
Xinwei MaoDepartment of Civil Engineering, Stony Brook University, Stony Brook, NY 11794, USA.ORCID 0000-0002-3070-8929
Baisuo ZhaoGraduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China.ORCID 0000-0002-2632-7939

Funding

Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences 1610042025005National Natural Science Foundation of China 31370158National Natural Science Foundation of China 31570110
6 · The paper itself

Abstract

Accurate gene expression quantification using reverse transcription quantitative PCR (RT-qPCR) requires stable reference genes (RGs) for reliable normalization. However, few studies have systematically identified RGs suitable for simultaneous high salt, alkaline, and high-temperature conditions. This study addresses this gap by evaluating the stability of eight candidate RGs in the anaerobic halophilic alkalithermophile

Indexed as

endogenous reference genesextremophilesNatranaerobius thermophiluspolyextreme conditionRT-qPCR

Identifiers

PMID40871225
PMCPMC12388727

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.