Evidence map›Paper›PMID 40869925›Full record

ArticleGenes2025

Comparative Transcriptome and Volatile Metabolome Analysis of

Ni Yang, Chaoli Xu, Yajun Liang, Juyun Zheng, Shiwei Geng, Fenglei Sun, Shengmei Li, Chengxia Lai, Mayila Yusuyin, Zhaolong Gong and 1 more

Abstract read
In one paragraph

Article in Genes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Ni YangNational Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China.
Chaoli XuNational Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China.
Yajun LiangNational Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China.
Juyun ZhengNational Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China.
Shiwei GengNational Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China.ORCID 0000-0003-4423-0695
Fenglei SunNational Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China.
Shengmei LiXinjiang Agricultural Vocational and Technical University, Changji 831100, China.ORCID 0000-0002-8597-8627
Chengxia LaiNational Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China.
Mayila YusuyinNational Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China.
Zhaolong GongNational Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China.
Junduo WangNational Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIn recent years, changes in climate conditions and long-term continuous cropping have led to the increased occurrence of Verticillium wilt in various cotton-growing regions, causing significant economic losses in cotton production. Research has shown that volatile substances are closely linked to plant disease resistance; however, studies on their roles in the response of cotton to Verticillium wilt, including their relationship with gene regulation, are limited.

methodsIn this study, the transcriptomes and metabolomes of Xinluzao 57 (a highly susceptible Verticillium wilt variety) and 192,868 (a highly resistant Verticillium wilt variety) were sequenced at different time points after inoculation with Verticillium wilt.

resultsA total of 21,911 commonly differentially expressed genes (DEGs) were identified within and between the materials, and they were clustered into eight groups. Significant annotations were made in pathways related to amino acids and anthocyanins. Metabolomics identified and annotated 26,200 volatile metabolites across nine categories. A total of 158 differentially accumulated metabolites (DAMs) were found within and between the materials; three clusters were identified, and the 10 metabolites with the most significant fold changes were highlighted. Weighted gene coexpression network analysis (WGCNA) revealed that 13 genes were significantly correlated with guanosine, 6 genes were correlated with 2-deoxyerythritol, and 32 genes were correlated with raffinose.

conclusionsOur results provide a foundation for understanding the role of volatile substances in the response of cotton to Verticillium wilt and offer new gene resources for future research on Verticillium wilt resistance.

Indexed as

Disease ResistanceGossypiumMetabolomePlant DiseasesTranscriptomeVerticilliumGene Expression ProfilingGene Expression Regulation, PlantVolatile Organic CompoundsVolatile Organic Compoundsamino acidsanthocyaninsGC–MSRNA-seqWGCNA

Identifiers

PMID40869925
PMCPMC12385826

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.