Evidence map›Paper›PMID 40869337›Full record

ArticleInternational journal of molecular sciences2025

PAM-Independent Cas12a Detection of Specific LAMP Products by Targeting Amplicon Loops.

Konstantin G Ptitsyn, Leonid K Kurbatov, Svetlana A Khmeleva, Daria D Morozova, Olga S Timoshenko, Elena V Suprun, Sergey P Radko, Andrey V Lisitsa

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Konstantin G PtitsynV.N. Orekhovich Institute of Biomedical Chemistry, 10 Pogodinskaya St., 119121 Moscow, Russia.ORCID 0000-0002-3197-6415
Leonid K KurbatovV.N. Orekhovich Institute of Biomedical Chemistry, 10 Pogodinskaya St., 119121 Moscow, Russia.
Svetlana A KhmelevaV.N. Orekhovich Institute of Biomedical Chemistry, 10 Pogodinskaya St., 119121 Moscow, Russia.ORCID 0000-0001-6224-4041
Daria D MorozovaV.N. Orekhovich Institute of Biomedical Chemistry, 10 Pogodinskaya St., 119121 Moscow, Russia.ORCID 0009-0000-2962-6152
Olga S TimoshenkoV.N. Orekhovich Institute of Biomedical Chemistry, 10 Pogodinskaya St., 119121 Moscow, Russia.
Elena V SuprunChemistry Faculty, M.V. Lomonosov Moscow State University, 1/3 Lenin Hills, 119991 Moscow, Russia.ORCID 0000-0002-2863-8562
Sergey P RadkoV.N. Orekhovich Institute of Biomedical Chemistry, 10 Pogodinskaya St., 119121 Moscow, Russia.ORCID 0000-0002-0519-1745
Andrey V LisitsaV.N. Orekhovich Institute of Biomedical Chemistry, 10 Pogodinskaya St., 119121 Moscow, Russia.

Funding

Program for Basic Research in the Russian Federation for a long-term period (2021-2030) 122030100170-5
6 · The paper itself

Abstract

A straightforward approach is suggested to selectively recognize specific products of loop-mediated isothermal amplification (LAMP) with the Cas12a nuclease without a need for a protospacer adjacent motif (PAM) in the sequence of LAMP amplicons (LAMPlicons). This strategy is based on the presence of single-stranded DNA loops in LAMPlicons and the ability of Cas12a to be

Indexed as

Bacterial ProteinsCRISPR-Associated ProteinsEndodeoxyribonucleasesMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesDNA, Single-StrandedRNA, Guide, CRISPR-Cas SystemsBacterial ProteinsCas12a proteinCRISPR-Associated ProteinsDNA, Single-StrandedEndodeoxyribonucleasesRNA, Guide, CRISPR-Cas Systemsamplicon loopsCas12aClavibacter speciesloop-mediated isothermal amplification

Identifiers

PMID40869337
PMCPMC12386220

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.