Evidence map›Paper›PMID 40869324›Full record

ArticleInternational journal of molecular sciences2025

Sulfur-Containing Heterocyclic Aromatic Hydrocarbons Alter Estrogen Metabolism and Cause DNA Damage and Apoptosis in Granulosa Cells.

Genevieve A Perono, Thane Tomy, Kara Loudon, Laiba Jamshed, Bianca Garlisi, Sylvia Lauks, Cielle Lockington, Celina Ruan, Gregg T Tomy, James J Petrik and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Genevieve A PeronoDepartment of Obstetrics and Gynecology, McMaster University, Hamilton, ON L8S 4L8, Canada.ORCID 0000-0003-1816-3433
Thane TomyCentre for Oil and Gas Research and Development, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
Kara LoudonCentre for Oil and Gas Research and Development, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
Laiba JamshedDepartment of Obstetrics and Gynecology, McMaster University, Hamilton, ON L8S 4L8, Canada.ORCID 0000-0002-8510-3740
Bianca GarlisiDepartment of Biomedical Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
Sylvia LauksDepartment of Biomedical Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
Cielle LockingtonDepartment of Biomedical Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
Celina RuanDepartment of Obstetrics and Gynecology, McMaster University, Hamilton, ON L8S 4L8, Canada.
Gregg T TomyCentre for Oil and Gas Research and Development, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
James J PetrikDepartment of Biomedical Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada.ORCID 0000-0001-7102-637X
Philippe J ThomasWildlife and Landscape Science Directorate, Environment and Climate Change Canada, National Wildlife Research Center, Ottawa, ON L1A 0H3, Canada.
Alison C HollowayDepartment of Obstetrics and Gynecology, McMaster University, Hamilton, ON L8S 4L8, Canada.ORCID 0000-0002-4343-5325

Funding

CIHR 461152Natural Sciences and Engineering Research Council CGS-DNatural Sciences and Engineering Research Council NSERC-USRANatural Sciences and Engineering Research Council RGPIN-05354-2019Natural Sciences and Engineering Research Council RGPIN-2020-06358University of Manitoba UM-URA
6 · The paper itself

Abstract

The expansion of the Alberta Oil Sands Region (AOSR) has increased the deposition of petroleum-derived chemicals into the surrounding environment. Among these, polycyclic aromatic compounds (PACs), including sulfur-containing heterocyclic hydrocarbons, have been detected in exposed local wildlife, yet the reproductive toxicity and genotoxicity of this suite of PACs remain largely unexplored. This study examined the effects of dibenzothiophene (DBT) and its alkylated congener, 2,4,7-trimethyldibenzothiophene (2,4,7-DBT), on estradiol (E2) synthesis and metabolism in granulosa cells (SIGCs). Cells were exposed to DBT or 2,4,7-DBT for 24 h at concentrations detected in AOSR wildlife tissues (0, 0.1, 1 and 10 nM). We measured the gene expression of markers involved in E2 synthesis, signaling and metabolism, E2 output via ELISA and E2 metabolite production via HPLC-MS/MS. Exposure to 2,4,7-DBT, but not DBT, shifted E2 metabolism towards 4-OHE2, a genotoxic E2 metabolite. DNA damage was assessed by γH2Ax expression, alongside DNA repair (

Indexed as

ApoptosisDNA DamageEstrogensGranulosa CellsHeterocyclic CompoundsSulfurThiophenesAnimalsEstradiolFemaledibenzothiopheneEstradiolEstrogensHeterocyclic CompoundsSulfurThiophenes2-OHE24-OHE2apoptosisDNA damageestrogen metabolismgranulosa cellovaryPAHpolycyclic aromatic compounds

Identifiers

PMID40869324
PMCPMC12386528

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.