Evidence map›Paper›PMID 40869118›Full record

ArticleInternational journal of molecular sciences2025

Multi-Layered Analysis of TGF-β Signaling and Regulation via DNA Methylation and microRNAs in Astrocytic Tumors.

Klaudia Skóra, Damian Strojny, Dawid Sobański, Rafał Staszkiewicz, Paweł Gogol, Mateusz Miller, Przemysław Rogoziński, Nikola Zmarzły, Beniamin Oskar Grabarek

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In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Klaudia SkóraDepartment of Neurological Rehabilitation, District Hospital of St. Padre Pio in Sędziszów Małopolski, 39-120 Sędziszów Małopolski, Poland.ORCID 0009-0006-8548-3321
Damian StrojnyCollegium Medicum, WSB University, 41-300 Dąbrowa Górnicza, Poland.ORCID 0009-0007-2324-3039
Dawid SobańskiCollegium Medicum, WSB University, 41-300 Dąbrowa Górnicza, Poland.ORCID 0000-0002-5525-6201
Rafał StaszkiewiczCollegium Medicum, WSB University, 41-300 Dąbrowa Górnicza, Poland.ORCID 0000-0002-0781-6534
Paweł GogolCollegium Medicum, WSB University, 41-300 Dąbrowa Górnicza, Poland.
Mateusz MillerDepartment of Neurology, Independent Public Healthcare Institution of the Ministry of Internal Affairs and Administration in Rzeszow, 35-111 Rzeszow, Poland.
Przemysław RogozińskiDental Office, Resdent Stomatology Rogozinscy, 35-326 Rzeszow, Poland.
Nikola ZmarzłyCollegium Medicum, WSB University, 41-300 Dąbrowa Górnicza, Poland.ORCID 0000-0002-7833-6167
Beniamin Oskar GrabarekCollegium Medicum, WSB University, 41-300 Dąbrowa Górnicza, Poland.ORCID 0000-0003-1633-7145

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Astrocytic tumors are a heterogeneous group of glial neoplasms characterized by marked differences in biological behavior and patient prognosis. Transforming growth factor-beta (TGF-β) signaling plays a pivotal role in astrocytoma pathogenesis; however, the extent and mechanisms of its epigenetic regulation remain poorly understood. This study aimed to investigate how promoter methylation and microRNA-mediated mechanisms regulate key genes within the TGF-β signaling pathway across various astrocytoma grades. Tumor tissue samples from 65 patients with WHO grade II-IV astrocytomas were analyzed using Affymetrix gene expression and microRNA microarrays. Promoter methylation of TGF-β signaling genes was assessed using methylation-specific polymerase chain reaction (MSP). Gene expression was validated by reverse transcription quantitative polymerase chain reaction (RT-qPCR), and protein levels were quantified using enzyme-linked immunosorbent assay (ELISA). MicroRNA targets were predicted using bioinformatic tools, and survival analyses were conducted using Kaplan-Meier and Cox regression models. Six genes-

Indexed as

AstrocytomaBrain NeoplasmsDNA MethylationMicroRNAsSignal TransductionTransforming Growth Factor betaAdultAgedEpigenesis, GeneticFemaleGene Expression Regulation, NeoplasticHumansMaleMiddle AgedPrognosisPromoter Regions, GeneticMicroRNAsTransforming Growth Factor betaastrocytic tumorsDNA methylationepigenetic regulationgene expression profilinggliomaMAPK1microRNAprognostic biomarkersSMAD proteinsTGF-β signaling

Identifiers

PMID40869118
PMCPMC12387059

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.