Evidence map›Paper›PMID 40869084›Full record

ArticleInternational journal of molecular sciences2025

Comprehensive Genetic and Molecular Characterization Confirms Hepatic Stellate Cell Origin of the Immortal Col-GFP HSC Line.

Larissa F Buitkamp, Thomas Liehr, Stefanie Kankel, Eva M Buhl, Katharina S Hardt, Diandra T Keller, Sarah K Schröder-Lange, Ralf Weiskirchen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Larissa F BuitkampInstitute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), RWTH University Hospital Aachen, D-52074 Aachen, Germany.
Thomas LiehrJena University Hospital, Institute of Human Genetics, Friedrich Schiller University, D-07747 Jena, Germany.ORCID 0000-0003-1672-3054
Stefanie KankelJena University Hospital, Institute of Human Genetics, Friedrich Schiller University, D-07747 Jena, Germany.
Eva M BuhlElectron Microscopy Facility, Institute of Pathology, RWTH University Hospital Aachen, D-52074 Aachen, Germany.ORCID 0000-0003-0627-9228
Katharina S HardtInstitute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), RWTH University Hospital Aachen, D-52074 Aachen, Germany.
Diandra T KellerInstitute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), RWTH University Hospital Aachen, D-52074 Aachen, Germany.ORCID 0009-0000-2266-3698
Sarah K Schröder-LangeInstitute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), RWTH University Hospital Aachen, D-52074 Aachen, Germany.ORCID 0000-0003-2265-3726
Ralf WeiskirchenInstitute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), RWTH University Hospital Aachen, D-52074 Aachen, Germany.ORCID 0000-0003-3888-0931

Funding

Deutsche Forschungsgemeinschaft WE2554/13-1, WE2554/15-1, and WE2554/17-1Deutsche Krebshilfe 70115581Interdisciplinary Centre for Clinical Research within the Faculty of Medicine at the RWTH Aachen University PTD 1-5
6 · The paper itself

Abstract

The immortal murine hepatic stellate cell line Col-GFP HSC was comprehensively characterized using genetic and molecular approaches. Short tandem repeat (STR) profiling and karyotyping combined with multiplex fluorescence in situ hybridization (M-FISH) confirmed the identity of the cell line and revealed no contamination. Col-GFP HSCs showed a near tetraploid karyotype. Additionally, next-generation sequencing (NGS) data, quantitative reverse transcription PCR, and Western blot analyses demonstrated robust expression of genes and proteins associated with hepatic stellate cells, including those involved in extracellular matrix remodeling and fibrogenic pathways. Phalloidin staining revealed filamentous actin patterns characteristic of stellate cells, providing additional support for their cytoskeletal organization and functional status. These findings provide strong evidence that the Col-GFP HSC cell line originates from hepatic stellate cells and can serve as a reliable in vitro model to study stellate cell biology and related pathophysiological processes.

Indexed as

Green Fluorescent ProteinsHepatic Stellate CellsAnimalsCell LineHigh-Throughput Nucleotide SequencingIn Situ Hybridization, FluorescenceKaryotypingMiceMicrosatellite RepeatsGreen Fluorescent Proteinscell authenticationCellosaurushepatic stellate cell(s)International Cell Line Authentication Committee (ICLAC)karyogramliverM-FISH analysisSTR profile

Identifiers

PMID40869084
PMCPMC12386721

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.