Evidence map›Paper›PMID 40867674›Full record

ArticleAnimals : an open access journal from MDPI2025

Analysis of the Role of KLF4 in the Regulation of Porcine Epidemic Diarrhea Virus Infection.

Haifei Wang, Yajing Zhou, Shanshen Gu, Mengke Feng, Jie Wang, Jian Jin, Xiaoguo Huang, Shenglong Wu, Wenbin Bao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animals : an open access journal from MDPI, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Haifei WangKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.ORCID 0000-0003-2424-9743
Yajing ZhouKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Shanshen GuKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Mengke FengKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Jie WangKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Jian JinKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Xiaoguo HuangJiangsu Fenghua Agricultural Group Co., Ltd., Changzhou 213148, China.
Shenglong WuKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Wenbin BaoKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.ORCID 0000-0001-5486-2253

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Porcine epidemic diarrhea virus (PEDV) is a primary pathogen responsible for viral diarrhea in swine. The identification of host resistance genes and key regulatory elements represents a critical prerequisite for developing novel control strategies. Krüppel-like factor 4 (KLF4), a multi-functional transcription factor, is known to regulate pathogenic infections; however, its specific roles in PEDV infection remain largely undefined. In this study, we found that KLF4 expression was upregulated following PEDV infection. Next, we constructed KLF4 knockout and overexpression cells and infected them with PEDV. The results show that viral RNA and protein expression levels and infectious viral titers were significantly enhanced in PEDV-infected KLF4 knockout cells compared to infected wild-type cells. In contrast, PEDV infection levels were significantly decreased in KLF4 overexpression cells relative to control cells. Transcriptomic analysis reveals that KLF4 significantly influences the expression of genes involved in key signaling pathways, including PI3K/Akt and MAPK. Overall, our findings elucidate the functional roles and underlying mechanisms of KLF4 during PEDV infection, offering valuable molecular targets for PEDV prevention and control.

Indexed as

gene editingKLF4PEDVtranscriptomevirus resistance

Identifiers

PMID40867674
PMCPMC12382836

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.