Liquiritigenin Restores Declined Oocyte Quality and Improves Embryo Development in Aged Mice.

Yelan Cai, Jiawei Ma, Haifeng Xu, Huamei Jian, Wenyi Gao, Man Wu, Bailin Liu, Ying Chen

Abstract read

In one paragraph

Article in Reproductive sciences (Thousand Oaks, Calif.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Yelan Cai *Institute of Medical Genetics, Wuxi Maternity and Child Health Care Hospital, Affiliated Women's Hospital of Jiangnan University, Wuxi, 214002, China.

Jiawei Ma *Institute of Medical Genetics, Wuxi Maternity and Child Health Care Hospital, Affiliated Women's Hospital of Jiangnan University, Wuxi, 214002, China.

Haifeng XuInstitute of Medical Genetics, Wuxi Maternity and Child Health Care Hospital, Affiliated Women's Hospital of Jiangnan University, Wuxi, 214002, China.

Huamei JianInstitute of Medical Genetics, Wuxi Maternity and Child Health Care Hospital, Affiliated Women's Hospital of Jiangnan University, Wuxi, 214002, China.

Wenyi GaoThe 904th, Hospital of Joint Logistic Support Force of PLA, Wuxi, 214002, China.

Man WuInstitute of Medical Genetics, Wuxi Maternity and Child Health Care Hospital, Affiliated Women's Hospital of Jiangnan University, Wuxi, 214002, China.

Bailin LiuInstitute of Medical Genetics, Wuxi Maternity and Child Health Care Hospital, Affiliated Women's Hospital of Jiangnan University, Wuxi, 214002, China.

Ying ChenInstitute of Medical Genetics, Wuxi Maternity and Child Health Care Hospital, Affiliated Women's Hospital of Jiangnan University, Wuxi, 214002, China. yingchen8@163.com.ORCID 0000-0001-5148-6504

Funding

Jiangsu Province's Key Provincial Talents Program SFY202103Top Medical Expert of Taihu Ren Cai Plan 2021-9Wuxi Municipal Health Commission Research Project M202303

6 · The paper itself

Abstract

Ovarian aging leads to a decline in oocyte quality and reduced reproductive potential, which is one of the main challenges faced by assisted reproductive technology (ART). Oxidative stress (OS) is a major contributor to this decline. In this study, we investigated the protective effects of natural flavonoid compound liquiritigenin (LQ) on oocyte maturation and embryo development in aged mice. The results showed that 20 μM LQ significantly improved the maturation rate of aged oocytes, restored spindle morphology, and enhanced fertilization and two-cell embryo development rates. Mechanism studies have found that LQ reduces the levels of reactive oxygen species (ROS) in oocytes and restores mitochondrial function, including distribution patterns and membrane potential. Additionally, LQ upregulated the protein expression of Sirtuin 1 (SIRT1) and nuclear factor E2-related factor 2 (NRF2) in the ovaries and oocytes of aging mice, as well as in the human ovarian granulosa tumor cell line (KGN). Although its mRNA level showed minimal change, it suggested that it might play a role through post-translational regulation. These results suggest that LQ protects aged oocytes from oxidative stress by activating the SIRT1/NRF2 signaling pathway, highlighting its potential as a natural antioxidant for alleviating ovarian aging and improving oocyte quality.

Indexed as

AgingAntioxidantsEmbryonic DevelopmentFlavanonesOocytesAnimalsFemaleHumansMiceNF-E2-Related Factor 2OvaryOxidative StressReactive Oxygen SpeciesSirtuin 1AntioxidantsFlavanonesliquiritigeninNfe2l2 protein, mouseNF-E2-Related Factor 2Reactive Oxygen SpeciesSirt1 protein, mouseSirtuin 1Aged oocyteLiquiritigeninNRF2Oxidative stressSIRT1

Identifiers

PMID40866779

DOI10.1038/s41598-024-65121-1

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.