ArticleDevelopmental cell2025
Large-scale single-cell profiling of stem cells identifies redundant regulators of shoot development and yield trait variation.
Article in Developmental cell, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed.
- The combination of morphogenic regulators BABY BOOM and GRF-GIF improves maize transformation efficiency and promotes leaf regeneration.The New phytologist · 2026Article
- Sample Preparation for Imaging-Based Spatial Transcriptomics in Rigid Plant Tissues (Roots, Shoots).Bio-protocol · 2026Article
- Brassinosteroid signaling regulates shoot apical meristem homeostasis via orchestrating cytokinin and WUSCHEL activity.Science advances · 2026Article
- Single-nucleus transcriptomics resolves multiple fate dynamics between inflorescence meristem and primary stem.Science advances · 2026Article
- The developing leaf of the wild grass Brachypodium distachyon at single-cell resolution.The Plant cell · 2026Article
- DNA repair under heat: DNA polymerase λ modulates heat stress-induced mutagenesis in plants.The Plant cell · 2026Article
- Gene expression imputation spatially resolves transcriptional programs in barley spike development.Nature plants · 2026Article
- Imputation integrates single-cell and spatial gene expression data to resolve transcriptional networks in barley shoot meristem development.Nature plants · 2026Article
- Spatial and single-cell expression analyses reveal complex expression domains in early wheat spike development.Genome biology · 2025Article
- A single-cell, spatial transcriptomic atlas of the Arabidopsis life cycle.Nature plants · 2025Article
- Exploring the untapped potential of single-cell and spatial omics in plant biology.The New phytologist · 2025Review
- Challenges of translating Arabidopsis insights into crops.The Plant cell · 2025Review
- A single-cell and spatial wheat root atlas with cross-species annotations delineates conserved tissue-specific marker genes and regulators.Cell reports · 2025Article
- Nucleoporin PNET1 coordinates mitotic nuclear pore complex dynamics for rapid cell division.Nature plants · 2025Article
- Meristem fate: to terminate, or not?Frontiers in plant science · 2025Review
- Harnessing Single-Cell and Spatial Transcriptomics for Crop Improvement.Plants (Basel, Switzerland) · 2024Review
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Abstract
Plant shoot stem cells generate organs essential for food, feed, and biofuels. However, plant single-cell analyses struggled to capture these rare cells or to detect stem cell regulators like CLAVATA3 and WUSCHEL. Here, we dissected stem cell-enriched shoot tissues from maize and Arabidopsis for single-cell RNA sequencing (scRNA-seq), and we optimized protocols to recover thousands of CLAVATA3- and WUSCHEL-expressing cells. A cross-species comparison identified deeply conserved stem cell regulators. We also profiled maize stem cell mutants, validated candidate regulators with spatial transcriptomics, and confirmed their roles in shoot development. These include a family of RNA-binding protein genes and two families of sugar kinase genes. Finally, we show how coupling large-scale single-cell profiling with allelic variation analysis in diverse maize germplasm serves as a valuable resource for identifying stem cell regulators significantly associated with grain yield components. Our discoveries advance the study of shoot stem cells and open avenues for rational crop engineering.
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